| Field | Specification |
|---|---|
| Target | |
| Alternative names | NCF1; NOXO2; SH3PXD1A; Neutrophil cytosol factor 1; NCF-1; 47 kDa autosomal chronic granulomatous disease protein; 47 kDa neutrophil oxidase factor; NCF-47K; Neutrophil NADPH oxidase factor 1; Nox organizer 2; Nox-organizing protein 2; SH3 |
| Gene ID | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human Neutrophil Cytosol Factor 1 around the phosphorylation site of Ser328. AA range:301-350 |
| Conjugate | |
| Concentration | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Scientific Background
The protein encoded by this gene is a 47 kDa cytosolic subunit of neutrophil NADPH oxidase. This oxidase is a multicomponent enzyme that is activated to produce superoxide anion. Mutations in this gene have been associated with chronic granulomatous disease. [provided by RefSeq, Jul 2008],
Product Overview
This polyclonal antibody, raised in Rabbit, recognizes p47-phox (phospho Ser328). It is validated for Western Blot, Immunohistochemistry, Immunofluorescence, ELISA.
Validated Applications
| Applications | Western Blot, Immunohistochemistry, Immunofluorescence, ELISA |
|---|---|
| Immunohistochemistry | 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications. |
Optimal dilutions should be determined by the end user for each application and sample type.
Immunogen
The antiserum was produced against synthesized peptide derived from human Neutrophil Cytosol Factor 1 around the phosphorylation site of Ser328. AA range:301-350
Specifications
| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse, Rat, Cow |
| Immunogen | The antiserum was produced against synthesized peptide derived from human Neutrophil Cytosol Factor 1 around the phosphorylation site of Ser328. AA range:301-350 |
| Concentration | 1 mg/mL |
| Subcellular Location | Cytoplasm, cytosol . Membrane ; Peripheral membrane protein ; Cytoplasmic side . |
| Swiss-Prot | P14598 |
| Gene ID | 653361 |
| Storage | -20°C/1 year |
Reactivity Notes
Validated reactivity: Human, Mouse, Rat, Cow. For other species, epitope conservation may allow cross-reactivity; confirm with appropriate controls.
Safety & Handling
This product is for research use only (RUO) and is not intended for diagnostic or therapeutic use. The formulation contains sodium azide; handle with standard laboratory precautions.
Related Products
Explore related antibodies and reagents for p47-phox (phospho Ser328) research from ELK Biotechnology, or contact us for alternative formats.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
