| Field | Specification |
|---|---|
| Mfr No | |
| Product Type | |
| Reporter | |
| Selection Marker | Blasticidin, Hygromycin, Puromycin, Zeocin |
| Shipping | |
| Species |
Background
The p52:RelB heterodimer is the principal effector of the non-canonical (alternative) NF-κB pathway. Unlike the canonical pathway, in which p50:RelA dimers respond to stimuli such as TNFα, the non-canonical pathway is activated when ligands of the TNF receptor superfamily, including lymphotoxin-β, CD40L, BAFF, RANKL, OX40L, and TWEAK, trigger processing of the p100 precursor into p52. The resulting p52:RelB dimer enters the nucleus to activate target genes that govern lymphoid organogenesis, B-cell maturation, and immune homeostasis. Homodimers of p52 or RelB alone lack transcriptional activity, so the heterodimer provides a specific readout of non-canonical NF-κB (NF-κB2) signaling.
Product Description & Applications
The p52:RelB Reporter Lentivirus is a transcription-factor reporter system that provides a sensitive fluorescent or luminescent readout of p52:RelB heterodimer activity in mammalian cells. It uses tandem repeats of consensus p52:RelB DNA-binding elements, designed to be activated preferentially by p52:RelB heterodimers over canonical p50:RelA dimers, coupled to a minimal promoter that drives the reporter. A constitutively expressed selection marker and optional secondary reporter support stable polyclonal reporter cell lines.
Stable lentiviral integration gives consistent reporter expression in dividing and post-mitotic cells, including primary and cryopreserved cultures, avoiding transient-transfection variability. Particles are purified by PEG precipitation and sucrose gradient centrifugation and transduce difficult-to-transfect cells, supporting study of the non-canonical NF-κB2 pathway with readout by microscopy, flow cytometry, or luminometry.
About This Product
This reporter lentivirus places a BFP2, d2GFP, EGFP, Firefly Luc, Gaussia Luc, GFP, GFP + Firefly Luc, mCherry, Renilla Luc, RFP, RFP + Firefly Luc reporter gene under the control of tandem consensus response elements specific for the p52:RelB heterodimer transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Blasticidin, Hygromycin, Puromycin, Zeocin) and/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.
Stable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.
Can't find the lentiviral construct you need, or want to adjust key design elements? Contact us to discuss custom LV design and optional add-ons.
Common customization requests
- Insert / payload: replace the gene/sequence, swap to a different isoform, add mutations, or optimize cloning features.
- Expression design: change promoter (e.g., CMV/EF1α/PGK), add enhancers, or adjust regulatory elements.
- Reporters: add/swap GFP/RFP/mCherry/luciferase (single or dual reporters where applicable).
- Selection markers: add/swap puromycin/blasticidin/neomycin or fluorescent selection options.
- Vector format: switch between OE, shRNA, CRISPR (sgRNA/Cas systems), or control vectors (where supported).
Add-ons you can request
- Control viruses: empty vector, non-targeting shRNA, reporter-only controls, or matched backbone controls.
- Packaging / format: concentration options, aliquoting, or custom fill volume for screening workflows.
- Documentation: construct map/sequence confirmation package (as available) and batch documentation.
What to include in your request
- Target cell type/model (cell line or primary cells) and intended readout (reporter, knockdown, OE, etc.)
- Insert sequence (FASTA) or reference ID, plus any required tags/mutations
- Promoter, reporter, and selection marker preferences
- Desired scale and preferred format (aliquots / concentration requests)
Email us at support@biohippo.com or use the Talk to a Scientist request form.
