| Field | Specification |
|---|---|
| Target | |
| Alternative names | PSME3; Proteasome activator complex subunit 3; 11S regulator complex subunit gamma; REG-gamma; Activator of multicatalytic protease subunit 3; Ki nuclear autoantigen; Proteasome activator 28 subunit gamma; PA28g; PA28gamma |
| Gene ID | |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | Purified recombinant human PA28γ protein fragments expressed in E.coli. |
| Conjugate | |
| Purification | |
| Concentration | |
| Formulation | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Scientific Background
The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits| 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. The immunoproteasome contains an alternate regulator, referred to as the 11S regulator or PA28, that replaces the 19S regulator. Three subunits (alpha, beta and gamma) o
Product Overview
This monoclonal antibody, raised in Mouse, recognizes PSME3. It is validated for Western Blot, Immunofluorescence.
Validated Applications
| Applications | Western Blot, Immunofluorescence |
|---|
Optimal dilutions should be determined by the end user for each application and sample type.
Immunogen
Purified recombinant human PA28γ protein fragments expressed in E.coli.
Specifications
| Host | Mouse |
|---|---|
| Clonality | Monoclonal |
| Isotype | IgG |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse, Dog, Pig |
| Immunogen | Purified recombinant human PA28γ protein fragments expressed in E.coli. |
| Purification | Affinity purification |
| Concentration | 1 mg/ml |
| Formulation | Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 50% glycerol. |
| UniProt Accession | Human: P61289; Mouse: P61290 |
| Gene ID | Human: 10197; Mouse: 19192 |
| Storage | -20°C for 1 year |
| Lead Time | Within one week |
Reactivity Notes
Validated reactivity: Human, Mouse, Dog, Pig. For other species, epitope conservation may allow cross-reactivity; confirm with appropriate controls.
Safety & Handling
This product is for research use only (RUO) and is not intended for diagnostic or therapeutic use. The formulation contains sodium azide; handle with standard laboratory precautions.
Related Products
Explore related antibodies and reagents for PSME3 research from Bioassay Technology Laboratory, or contact us for alternative formats.
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