| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human PAFAH1B2 recombinant protein (Position: D5-D190) was used as the immunogen for the PAFAH1B2 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
PAFAH1B2 Antibody / Platelet-activating factor acetylhydrolase IB subunit beta is a anti-PAFAH1B2 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasm.
Key elements and design rationale
- Target: PAFAH1B2
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, ELISA
Biological background
PAFAH1B2 acts as a cytoplasmic serine hydrolase that removes acetyl groups from PAF, reducing its proinflammatory activity. Beyond lipid metabolism, it contributes to neuronal migration and brain development through its association with LIS1, which regulates microtubule dynamics and dynein-mediated transport. Mutations or dysfunction in components of this complex are associated with lissencephaly and neurodevelopmental defects.
The PAFAH1B2 antibody is widely used in neuroscience, lipid signaling, and developmental biology research to investigate lipid hydrolysis, microtubule regulation, and neuronal migration. Western blot analysis identifies a 26 kilodalton band corresponding to PAFAH1B2, while immunofluorescence reveals cytoplasmic localization that overlaps with microtubule structures in neurons. This antibody enables detection of the catalytic subunit in both neuronal and non-neuronal tissues, supporting studies of phospholipid turnover and signaling.
PAFAH1B2 also plays roles in inflammation and oxidative stress by modulating PAF levels. Abnormal expression has been linked to neuroinflammation and ischemic injury, as well as cancer metabolism where lipid mediators regulate proliferation. The PAFAH1B2 antibody provides a reliable reagent for exploring these processes.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
