| Field | Specification |
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| Species | |
| Applications | |
| Sample type(s) | Chemical compounds |
| Detection method | |
| Plate format | |
| Assay time | |
| Storage | |
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Overview
For quantitative determination of gastrointestinal (GI) membrane permeability of test compounds. The assay uses PAMPA for signal readout. Compatible sample input includes Chemical compounds. Typical stated assay timing is Assay takes 20 hr, hands-on time 1 hr.
Key elements and design rationale
- Readout format: PAMPA supports plate-based signal acquisition and consistent comparison across matched samples.
- Sample compatibility: The stated sample scope includes Chemical compounds, which is useful when aligning matrix type with calibration and control design.
- Workflow timing: The listed assay time of Assay takes 20 hr, hands-on time 1 hr helps frame batch planning, replicate handling, and plate throughput.
- Feature emphasis: Convenient. Includes all necessary equipment to run a PAMPA plate.
Additional feature notes highlight Simple and low-cost. Procedure is easy to follow and more affordable than cell-based permeability assays; High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Available format information for this listing includes 96 Tests.
Biological background
This product is centered on measurement of parallel artificial membrane permeability assay (pampa) within the matrices described for the assay. In practice, datasets from this type of format are typically interpreted by comparing relative signal, activity, or abundance across matched control and experimental groups rather than relying on a single value in isolation. Careful alignment of sample matrix, incubation window, and calibration strategy is important when comparing results across plates, operators, or study days.
More details
MEMBRANE PERMEABILITY is an important characteristic to determine for evaluating compounds as potential drug candidates. Drugs often need to cross cell membranes in order to reach their target of action and this makes a compound’s ability to passively cross these membranes an important characteristic to evaluate. Permeability can be evaluated by cell-based methods; however, these methods are often expensive and time consuming. Parallel Artificial Permeability Assays (PAMPA) offer researchers a quick, inexpensive method of evaluating the gastrointestinal (GI) permeability of test compounds. BioAssay Systems’ PAMPA Kit provides all the necessary components to run a Parallel Artificial Permeability Assay.
Detection method
PAMPA.
Procedures and timing
Stated procedure or timing information: Assay takes 20 hr, hands-on time 1 hr.
Research relevance and current trends
- Plate-based quantification and side-by-side group comparison remain central use cases for this assay format.
- The product notes emphasize multi-sample throughput, making it relevant for screening-oriented and larger batch comparison studies.
- Short assay timing and plate compatibility support time-course or repeated-measure collection plans when handling is kept consistent.
Common research applications
- Quantify parallel artificial membrane permeability assay (pampa) in chemical compounds by PAMPA readout.
- Compare treatment or phenotype groups using matched chemical compounds handling.
- Monitor time-course or pre/post changes in chemical compounds across study conditions.
Interpretation is usually strongest when signal changes are assessed alongside matrix-matched controls, replicate agreement, and the assay's stated analytical window.
Notes for experimental interpretation
- Matrix composition, background signal, and sample handling can influence apparent response; compare like-with-like whenever possible.
- Use appropriate blanks, controls, and replicate wells to distinguish biological differences from plate, reagent, or handling variability.
What are the Permeability Controls?
The High, Medium, and Low Permeability Controls are 10mM stock solutions of Chloramphenicol, Diclofenac, and Theophylline, respectively, in DMSO.
What other materials are required to run the assay?
Pipetting devices, DMSO, PBS, UV Plates (Cat # P96UV), and an absorbance plate reader capable of absorbance spectra.
What is the membrane of the donor plate?
The donor plate membrane is a Hydrophobic PVDF membrane with a pore size of 0.45 µm.
My test compounds aren’t soluble at 500 µM in PBS. Can I use higher concentrations of DMSO in the PAMPA assay?
We recommend against using higher concentrations of DMSO in the PAMPA assay. The spectrophotometric background in the Acceptor solution will increase with higher DMSO concentrations, and the higher concentration of DMSO may alter the membrane and affect the result.
If your compound(s) is/are insoluble in PBS with 5% DMSO, clarify the solution by centrifugation at 13,000xg for 10 minutes then use the supernatant for the Donor solution. The Donor solution is used in place of “500 µM Test Compound”. Since some of the compound was centrifuged out, the new concentration is unknown, so it is instead referred to as Donor Solution.
For the Equilibrium Standard, mix 80 µL of the new Donor Solution with 120 µL of PBS.
For laboratories requiring additional technical capacity, we provide scientific support services including assay execution, method guidance, product sourcing, and customization to align the assay with specific experimental objectives. If you need assistance selecting the appropriate kit configuration, adapting the workflow to your application, or identifying related research services, please click Talk to a Scientist, email support@biohippo.com, or review our Research Services; a member of our scientific team will follow up with recommendations tailored to your study.
