| Field | Specification |
|---|---|
| Mfr No | |
| Product Type | |
| Reporter | |
| Selection Marker | Puromycin, Blasticidin |
| Shipping | |
| Species |
Background
PAX8 is a paired-box transcription factor essential for the development of the thyroid gland, kidney, and Müllerian (female reproductive) system, where it controls organ-specific gene programs. In the thyroid, PAX8 cooperates with other factors to activate genes such as thyroperoxidase. PAX8 also remains a marker of epithelial tumors arising in the thyroid, kidney, thymus, and female genital tract, and it can drive proliferation by cooperating with partners such as HIF2α to upregulate cell-cycle genes. A PAX8 reporter detects activity through tandem PAX8 DNA-binding elements derived from the thyroperoxidase promoter, providing a readout of PAX8 transcriptional function in development and cancer research.
Product Description & Applications
The PAX8 Reporter Lentivirus is a transcription-factor reporter system for sensitive fluorescent or luminescent detection of human and mouse PAX8 activity. It contains tandem repeats of consensus PAX8 DNA-binding elements, derived from the thyroperoxidase promoter, coupled to a minimal TATA-box promoter and an upstream enhancer that maximizes signal-to-noise to drive a reporter gene (d2GFP, EGFP, GFP, mCherry, RFP, or firefly luciferase). A constitutively expressed selection marker (puromycin or blasticidin) supports stable polyclonal cell line generation. Stable lentiviral integration provides consistent reporter expression in dividing and post-mitotic cells, including primary and cryopreserved material, and allows readout by microscopy, flow cytometry, or luminometry. It is used to study PAX8 transcriptional activity in developmental biology and renal research. Supplied as purified lentiviral particles.
About This Product
This reporter lentivirus places a d2GFP, EGFP, Firefly Luc, GFP, mCherry, RFP reporter gene under the control of tandem consensus response elements specific for the PAX8 transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Puromycin, Blasticidin) and/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.
Stable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.
Can't find the lentiviral construct you need, or want to adjust key design elements? Contact us to discuss custom LV design and optional add-ons.
Common customization requests
- Insert / payload: replace the gene/sequence, swap to a different isoform, add mutations, or optimize cloning features.
- Expression design: change promoter (e.g., CMV/EF1α/PGK), add enhancers, or adjust regulatory elements.
- Reporters: add/swap GFP/RFP/mCherry/luciferase (single or dual reporters where applicable).
- Selection markers: add/swap puromycin/blasticidin/neomycin or fluorescent selection options.
- Vector format: switch between OE, shRNA, CRISPR (sgRNA/Cas systems), or control vectors (where supported).
Add-ons you can request
- Control viruses: empty vector, non-targeting shRNA, reporter-only controls, or matched backbone controls.
- Packaging / format: concentration options, aliquoting, or custom fill volume for screening workflows.
- Documentation: construct map/sequence confirmation package (as available) and batch documentation.
What to include in your request
- Target cell type/model (cell line or primary cells) and intended readout (reporter, knockdown, OE, etc.)
- Insert sequence (FASTA) or reference ID, plus any required tags/mutations
- Promoter, reporter, and selection marker preferences
- Desired scale and preferred format (aliquots / concentration requests)
Email us at support@biohippo.com or use the Talk to a Scientist request form.
