Paxilline

Product details

• Chemical name: (2R,4bS,6aS,12bS,12cR,14aS)-5,6,6a,7,12,12b,12c,13,14,1 4a-Decahydro-4b-hydroxy-2-(1-hydroxy-1-methylethyl)-12b ,12c-dimethyl-2H-pyrano[2'',3'':5',6']benz[1',2':6,7]in deno[1,2-b]indol-3(4bH)-one.
• CAS number: 57186-25-1
• Molecular formula: C27H33NO4.
• Purity: >98% (HPLC)
• Concentration: 10-100 nM.
• Form: Lyophilized powder.
• Solubility: DMSO or acetone. Centrifuge all product preparations before use (10000 x g 5 min).
• Reconstitution: Centrifuge the vial before adding solvent (10,000 x g for 5 minutes) to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity
• Shipping: Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
• Target: KCa K+ channels, SERCA pump
• Source: Natural
• Activity: Paxilline is a selective blocker of high-conductance Ca2+-activated (Maxi-K) K+ channels.

Scientific background

Paxilline is a selective blocker of KCa1.1 (high-conductance Ca2+-activated K+, BK) channels. It belongs to a set of tremorgenic indole alkaloids which are lipid soluble and also include Penitrem A and Verruculogen.2Paxilline enhanced the electrically induced twitch contractions in guinea pig ileum, without influencing the contractions provoked by exogenous acetylcholine.1 Paxilline was shown to inhibit the peptidyle toxin resistant combination a+b4 channels (for more background see 3) expressed in CHO cells.4 It was shown to be as potent as Iberiotoxin in action potential broadening in hippocampus and amygdala.4,5Paxilline also inhibits intracellular Ca2+-ATPases (SERCA) with IC50s between 5 and 10 µM6.

Lead time: 1-2 Business Days

Country of origin: Israel/IL

Applications key

Application key: FC- Flow cytometry, IFC- Indirect flow cytometry, IHC- Immunohistochemistry,LCI- Live cell imaging, Calcium imaging assay,Cell survival assay, Electrophysiology, Neurite outgrowth assay.

Bioassay tested: Yes

• SDS (PDF)

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