| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human PCBD1 recombinant protein (Position: M1-T105) was used as the immunogen for the PCBD1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
PCBD1 Antibody / Pterin-4 alpha-carbinolamine dehydratase is a anti-PCBD1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), ELISA with listed reactivity in Human, Mouse.
Key elements and design rationale
- Target: PCBD1
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, ICC, IF, ELISA
Biological background
In its enzymatic role, PCBD1 catalyzes the dehydration of pterin-4 alpha-carbinolamine to quinonoid dihydrobiopterin, a step required for BH4 cofactor regeneration. Independently, as a dimerization cofactor, it stabilizes the DNA-binding form of HNF1A, influencing transcription of genes involved in glucose metabolism and hepatic differentiation. This dual functionality links metabolic and transcriptional control mechanisms within hepatocytes, endocrine tissues, and the central nervous system.
The PCBD1 antibody is widely used in enzymology, metabolic, and transcriptional biology research to study BH4 metabolism, amino acid hydroxylation, and HNF1A-dependent transcription. Western blot analysis identifies a 12 kilodalton band corresponding to PCBD1, while immunofluorescence reveals cytoplasmic and nuclear localization depending on cellular context. This antibody supports the investigation of enzyme regulation and transcriptional cofactor dynamics.
Mutations in PCBD1 cause hyperphenylalaninemia and transient neonatal diabetes due to impaired cofactor recycling and altered HNF1A transcriptional activity. The PCBD1 antibody is an essential reagent for exploring metabolic diseases, neurotransmitter synthesis, and transcriptional regulation.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
