| Field | Specification |
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| Mfr No | |
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| Host | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human PCGF6 was used as the immunogen for the PCGF6 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
PCGF6 Antibody / Polycomb group RING finger protein 6 is a anti-PCGF6 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS) with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: PCGF6
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, FACS
Biological background
PCGF6 contains a RING finger domain responsible for interacting with the ubiquitin ligases RING1A/B, promoting histone modification that represses gene transcription. Through association with the transcriptional repressors E2F6 and MGA, PCGF6 regulates genes involved in cell proliferation, germline maintenance, and differentiation. It is expressed in embryonic stem cells, germ cells, and certain somatic tissues, where it contributes to pluripotency and developmental gene silencing. PCGF6 activity ensures appropriate repression of lineage-specific genes during embryogenesis and germ cell development.
The PCGF6 antibody is used in epigenetics, developmental biology, and transcriptional regulation studies to investigate Polycomb complex function and chromatin remodeling. Western blot analysis detects a 37 kilodalton band corresponding to PCGF6, and immunofluorescence reveals nuclear punctate staining associated with chromatin regions. This antibody enables researchers to track PRC1.6 distribution and assess its role in transcriptional repression and stem cell identity.
Aberrant expression of PCGF6 has been implicated in cancer and developmental disorders. Overexpression supports cell proliferation and transformation, while depletion can impair germ cell specification and induce differentiation. By regulating histone ubiquitination and chromatin architecture, PCGF6 acts as a molecular link between epigenetic control and transcriptional silencing. The PCGF6 antibody provides a dependable tool for studying these mechanisms in both stem cell and cancer research.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
