| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human PDE4B recombinant protein (Position: M1-T736) was used as the immunogen for the PDE4B antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
PDE4B Antibody / Phosphodiesterase 4B is a anti-PDE4B Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), ELISA with listed reactivity in Human, Mouse.
Key elements and design rationale
- Target: PDE4B
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, ELISA
Biological background
PDE4B is encoded by the PDE4B gene on human chromosome 1p31.3. The protein exists in multiple isoforms ranging from 70 to 100 kilodaltons, all containing a catalytic domain, upstream conserved regions (UCR1 and UCR2), and regulatory sequences that determine localization and activation. PDE4B localizes to cytoplasmic and perinuclear regions and interacts with signaling complexes via A-kinase anchoring proteins (AKAPs).
The PDE4B antibody identifies characteristic isoforms in western blot. Functionally, PDE4B hydrolyzes cAMP generated by G protein-coupled receptor activation, shaping responses to neurotransmitters, hormones, and immune stimuli. It regulates protein kinase A (PKA) activity and downstream transcription factors such as CREB.
In immune cells, PDE4B modulates cytokine production and inflammatory responses. Inhibition of PDE4B enhances cAMP signaling, reducing pro-inflammatory cytokines like TNF-alpha and IL-6. In the central nervous system, PDE4B regulates neuronal excitability, memory, and emotional processing. Genetic and pharmacologic studies implicate PDE4B dysfunction in depression, schizophrenia, and addiction.
PDE4B is a therapeutic target for anti-inflammatory and neuropsychiatric drugs. Selective inhibitors such as rolipram and roflumilast have been developed to modulate cAMP signaling.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
