| Field | Specification |
|---|---|
| Mfr No | |
| Accession Number | |
| Product Type | |
| Reporter | |
| Selection Marker | GFP (constitutively expressed), RFP (constitutively expressed), Hygromycin, Zeocin, Puromycin, Blasticidin |
| Shipping | |
| Species |
Background
PDE7A is a cAMP-specific phosphodiesterase that hydrolyzes the second messenger cyclic AMP and thereby limits signaling through the cAMP/PKA/CREB pathway. CREB (cAMP response element-binding protein) is a transcription factor that, when phosphorylated downstream of elevated cAMP, binds cAMP response elements to activate target genes. In T lymphocytes, cAMP levels influence activation and proliferation, and PDE7A helps set the threshold for these responses by controlling cAMP turnover. Because of this role, PDE7A is studied as a regulator of immune cell function and as a potential drug target, and CREB-driven reporters provide a quantitative readout of how PDE7A activity shapes cAMP-dependent transcription.
Product Description & Applications
The PDE7A/CREB Reporter Lentivirus is an immunotherapy reporter system for studying PDE7A function within the cAMP/CREB pathway. A receptor lentivirus constitutively expresses human PDE7A with antibiotic selection, while a reporter lentivirus carries CREB response elements driving a dual reporter, secreted Gaussia luciferase and a fluorescent protein. Sequential transduction and selection generates a dual-stable cell line in which PDE7A activity modulates intracellular cAMP and the resulting CREB-dependent reporter output.
Secreted Gaussia luciferase accumulates in conditioned media, enabling kinetic sampling without cell lysis, while fluorescence supports microscopy and flow cytometry. The VSV-G-pseudotyped particles are purified by PEG precipitation and sucrose gradient centrifugation and transduce difficult-to-transfect cells, including primary and thawed cultures, supporting T-cell activation assays and immune signaling research.
About This Product
This 2-vial immunotherapy reporter system consists of a Vial 1 Receptor Lentivirus encoding human PDE7A under a constitutive promoter with antibiotic selection, and a Vial 2 Reporter Lentivirus encoding tandem NFAT (or NF-κB) response elements driving a dual reporter (GFP, GFP-P2A-GLuc, GLuc, GLuc-P2A-GFP, GLuc-P2A-RFP, RFP, RFP-P2A-GLuc). Sequential transduction and selection generates a dual-stable effector cell line that responds quantitatively to receptor stimulation with a ratiometric fluorescent + bioluminescent readout.
Secreted Gaussia luciferase (where included) accumulates in conditioned media, enabling kinetic sampling without cell lysis. The combined fluorescent and luminescent outputs allow parallel microscopy-based visualization and plate-reader luminometry from the same cell population — providing assay redundancy and flexibility for potency testing formats compliant with regulatory expectations for cell-based functional assays.
Can't find the lentiviral construct you need, or want to adjust key design elements? Contact us to discuss custom LV design and optional add-ons.
Common customization requests
- Insert / payload: replace the gene/sequence, swap to a different isoform, add mutations, or optimize cloning features.
- Expression design: change promoter (e.g., CMV/EF1α/PGK), add enhancers, or adjust regulatory elements.
- Reporters: add/swap GFP/RFP/mCherry/luciferase (single or dual reporters where applicable).
- Selection markers: add/swap puromycin/blasticidin/neomycin or fluorescent selection options.
- Vector format: switch between OE, shRNA, CRISPR (sgRNA/Cas systems), or control vectors (where supported).
Add-ons you can request
- Control viruses: empty vector, non-targeting shRNA, reporter-only controls, or matched backbone controls.
- Packaging / format: concentration options, aliquoting, or custom fill volume for screening workflows.
- Documentation: construct map/sequence confirmation package (as available) and batch documentation.
What to include in your request
- Target cell type/model (cell line or primary cells) and intended readout (reporter, knockdown, OE, etc.)
- Insert sequence (FASTA) or reference ID, plus any required tags/mutations
- Promoter, reporter, and selection marker preferences
- Desired scale and preferred format (aliquots / concentration requests)
Email us at support@biohippo.com or use the Talk to a Scientist request form.
