| Field | Specification |
|---|---|
| Mfr No | |
| Accession Number | |
| Product Type | |
| Promoter | |
| Reporter | |
| Selection Marker | Puromycin, Blasticidin, N/A |
| Shipping | |
| Species |
Background
PDGF-D (platelet-derived growth factor D) is a member of the PDGF family of growth factors. It is secreted as a latent protein that requires proteolytic removal of its CUB domain to release the active growth-factor domain, which then signals primarily through the PDGF receptor beta. PDGF-D signaling stimulates the proliferation, migration, and survival of mesenchymal cells such as fibroblasts and smooth muscle cells, and contributes to angiogenesis, wound healing, and tissue remodeling. Aberrant PDGF-D activity has been linked to fibrosis, atherosclerosis, and tumor progression, where it can promote a supportive microenvironment, making PDGF-D a target of interest in cancer and cell biology research.
Product Description & Applications
The PDGFD ORF cDNA Lentivirus delivers the open reading frame of human PDGF-D (transcript variant 1) for stable overexpression in mammalian cells. A CMV promoter drives expression of PDGF-D fused to a C-terminal V5 tag, with a fluorescent reporter (GFP or RFP) and a drug selection marker (puromycin or blasticidin) separated from the cDNA by self-cleaving peptides for independent translation. In some configurations a PGK promoter drives the reporter and selection marker.
The vectors are optimized for high expression and reliable genome integration, enabling long-term stable cell lines. ORF cloning accuracy is confirmed by sequencing and protein expression validated by transient transfection. Particles efficiently transduce difficult-to-transfect cells, including primary and thawed cells.
About This Product
This ORF cDNA lentivirus enables stable overexpression of PDGFD (NCBI Accession: NM_025208) in mammalian cells via a third-generation, VSV-G pseudotyped delivery system. The ORF cDNA is fused to a C-terminal epitope tag (V5, Myc, or HA) and expressed under a strong constitutive promoter (CMV). Reporter and selection marker components (GFP, RFP; Puromycin, Blasticidin) are co-expressed via self-cleaving P2A peptides, enabling independent protein production without fusion-tag artifacts.
Ultra-purification by PEG precipitation and sucrose gradient centrifugation yields high-titer particles suitable for primary cells, suspension cultures, and stem cells. Stable polyclonal cell lines are established within 10–14 days by antibiotic selection or FACS sorting. For in vivo applications, the serum-free formulation and VSV-G envelope support direct administration or further concentration for stereotactic injection.
Can't find the lentiviral construct you need, or want to adjust key design elements? Contact us to discuss custom LV design and optional add-ons.
Common customization requests
- Insert / payload: replace the gene/sequence, swap to a different isoform, add mutations, or optimize cloning features.
- Expression design: change promoter (e.g., CMV/EF1α/PGK), add enhancers, or adjust regulatory elements.
- Reporters: add/swap GFP/RFP/mCherry/luciferase (single or dual reporters where applicable).
- Selection markers: add/swap puromycin/blasticidin/neomycin or fluorescent selection options.
- Vector format: switch between OE, shRNA, CRISPR (sgRNA/Cas systems), or control vectors (where supported).
Add-ons you can request
- Control viruses: empty vector, non-targeting shRNA, reporter-only controls, or matched backbone controls.
- Packaging / format: concentration options, aliquoting, or custom fill volume for screening workflows.
- Documentation: construct map/sequence confirmation package (as available) and batch documentation.
What to include in your request
- Target cell type/model (cell line or primary cells) and intended readout (reporter, knockdown, OE, etc.)
- Insert sequence (FASTA) or reference ID, plus any required tags/mutations
- Promoter, reporter, and selection marker preferences
- Desired scale and preferred format (aliquots / concentration requests)
Email us at support@biohippo.com or use the Talk to a Scientist request form.
