| Field | Specification |
|---|---|
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Applications | |
| Conjugate | |
| Concentration | |
| Storage | |
| Storage buffer | |
| Shipping | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
CD11b is a 170 kD glycoprotein also known as αM integrin, Mac-1 α subunit, Mol, CR3, and Ly-40. CD11b is a member of the integrin family, primarily expressed on granulocytes, monocytes/macrophages, dendritic cells, NK cells, and subsets of T and B cells. CD11b non-covalently associates with CD18 (β2 integrin) to form Mac-1. Mac-1 plays an important role in cell-cell interaction by binding its ligands ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4 (CD242), iC3b, and fibrinogen.
Validated Applications & Recommended Dilutions
| Application | Recommended Dilution |
|---|---|
| Flow Cytometry | See product datasheet |
Antibody Specifications
| Target | CD11b |
|---|---|
| Host Species | Rat |
| Clonality | Monoclonal |
| Isotype | Rat IgG2b, κ |
| Conjugate | PE |
| Clone Number | M1/70 |
| Purification | See datasheet |
| Concentration | 5 μL/Test |
Immunogen
Recombinant protein fragment; see full datasheet. UniProt: P05555;P11215.
Species Reactivity & Cross-Reactivity
Validated for: Human, Mouse. Verify suitability in your sample type prior to use. For species or applications not listed in the datasheet, consult our technical team before purchase.
Storage & Handling
This product can be stored at 2-8°C for 12 months. Please protected from prolonged exposure to light and do not freeze.
Storage Buffer: Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Related Products
Browse additional antibodies targeting CD11b and complementary reagents — including secondary antibodies, blocking buffers, and detection kits — available through BioHippo.
This antibody is validated for flow cytometry applications. For intracellular targets, fix and permeabilize cells using a validated fixation/permeabilization kit (e.g., BD Cytofix/Cytoperm or similar) before staining. For surface markers, stain live or fixed cells without permeabilization. Always include a matched isotype control (Rat IgG2b, κ, Rat origin) at the same protein concentration as the test antibody to establish non-specific binding thresholds. Recommended starting dilution/concentration: see datasheet.
This antibody has been experimentally validated in: Human, Mouse. Cross-reactivity with other species has not been systematically evaluated. If your sample species is not listed, assess immunogen sequence homology to your target species ortholog. The immunogen used is: "" — higher sequence identity between species increases the probability of cross-reactivity, but experimental validation with appropriate controls is necessary before drawing scientific conclusions.
This product can be stored at 2-8°C for 12 months. Please protected from prolonged exposure to light and do not freeze. Storage Buffer: Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.. To maintain antibody activity, avoid repeated freeze-thaw cycles. Aliquot into single-use volumes before first freeze. If working with the antibody frequently, keep a working stock at 4°C for up to 4 weeks — add 0.02% sodium azide to the working stock as a bacteriostatic agent if azide is not already present in the buffer. Monitor performance against a validated positive control when transitioning between storage aliquots or lots.
This monoclonal antibody (clone M1/70) provides highly consistent, epitope-specific recognition of CD11b, making it ideal for quantitative assays, multiplex panels, and studies requiring strict reproducibility across experiments and independent laboratories. Unlike polyclonal preparations, each production batch from a single hybridoma clone generates antibody with identical epitope specificity, affinity, and isotype — ensuring reliable signal comparison in longitudinal studies or multi-site collaborations. When citing this antibody in publications, include the clone designation (clone M1/70) to enable third-party reproducibility verification.
Customization & Add-ons: Can't find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
M2 macrophage-derived exosomes delivering haptoglobin and interleukin-10 plasmids for synergistic therapy of intracerebral hemorrhage (2026) Bioactive Materials. 10.1016/j.bioactmat.2026.01.047
Inhalable Chemotactic Liposome for Targeted Modulation of Pulmonary Pre-Metastatic Niche (2025) ADVANCED FUNCTIONAL MATERIALS. 10.1002/adfm.202419583
Photosynthetic Plant-Derived Nanovesicles Precisely Amplify Photodynamic Effect by Light-Activated Oxygen Generation for Enhanced Cancer Photoimmunotherapy (2025) ACS Nano. 10.1021/acsnano.5c13350
A Hybrid Manganese Nanoparticle Simultaneously Eliminates Cancer Stem Cells and Activates STING Pathway to Potentiate Cancer Immunotherapy (2025) ACS Nano. 10.1021/acsnano.5c00322
Surface hydrophobicity and rigidity determines protein corona on orally delivered nanoparticles treating colitis (2026) Nature Communications. 10.1038/s41467-026-70453-9
Cell surface patching via CXCR4-targeted nanothreads for cancer metastasis inhibition (2024) Nature Communications. 10.1038/s41467-024-47111-z
Targeting IGF1R Overcomes Armored and Cold Tumor Microenvironment and Boosts Immune Checkpoint Blockade in Triple-Negative Breast Cancer (2025) Advanced Science. 10.1002/advs.202501341
Multifunctional Framework Nucleic Acid Vehicle for Antibiotic Sensitization and Treatment of Methicillin-Resistant Staphylococcus aureus (2025) Aggregate. 10.1002/agt2.70059
Armouring Bacillus Calmette-Guérin with metal-phenolic networks for bladder cancer therapy (2026) CHEMICAL ENGINEERING JOURNAL. 10.1016/j.cej.2026.174139
PD1 cell membrane-coated Ru(ll)-Pt(lV) complex nanoparticles inhibit liver metastatic melanoma via cascade immune activation (2026) JOURNAL OF NANOBIOTECHNOLOGY. 10.1186/s12951-026-04167-x
Clostridium butyricum alleviates multiple myeloma by remodeling the bone marrow microenvironment and inhibiting PI3K/AKT pathway through the gut?bone axis (2026) Gut Microbes. 10.1080/19490976.2025.2609455
PROTAC-based nanoantigens promote cross-presentation and trafficking of dendritic cell vaccine for enhanced antitumor efficacy (2025) Journal for ImmunoTherapy of Cancer. 10.1136/jitc-2025-012405
Multifunctional self-cascade nanoplatform for efficient immunotherapy through synergizing pyroptosis and self-augmented activation of cGAS-STING pathway (2025) JOURNAL OF COLLOID AND INTERFACE SCIENCE. 10.1016/j.jcis.2025.139423
A POSS-Based Metal-Phenolic Network with Coupled Hardness and Softness for Photodynamic-Immunotherapy of Triple-Negative Breast Cancer (2024) ACS Materials Letters. 10.1021/acsmaterialslett.4c00661
CpG-Conjugated Silver Nanoparticles as a Multifunctional Nanomedicine to Promote Macrophage Efferocytosis and Repolarization for Atherosclerosis Therapy (2023) ACS Applied Materials & Interfaces. 10.1021/acsami.3c11227
Cucurbitacin B stimulates PD-1 immunotherapy response in malignant breast cancer by covalent targeting MTCH2 (2025) PHYTOMEDICINE. 10.1016/j.phymed.2025.157017
Engineered Carrier-Free Nanosystem-Induced In Situ Therapeutic Vaccines for Potent Cancer Immunotherapy (2024) ACS Applied Materials & Interfaces. 10.1021/acsami.4c09925
ANXA3-rich exosomes derived from tumor-associated macrophages regulate ferroptosis and lymphatic metastasis of laryngeal squamous cell carcinoma (2024) Cancer Immunology Research. 10.1158/2326-6066.CIR-23-0595
Reversing cancer immunosuppression via K+ capture and repolarization of tumor-associated macrophages (2025) Nanoscale Horizons. 10.1039/D5NH00050E
LILRB4 shapes an immunosuppressive microenvironment to drive cervical cancer progression through tumor-infiltrating myeloid cell expansion and CD8+ T-cell suppression (2026) CELLULAR AND MOLECULAR LIFE SCIENCES. 10.1007/s00018-026-06121-4
In Vitro and In Vivo Dendritic Cell Immune Stimulation Effect of Low Molecular Weight Fucoidan from New Zealand Undaria pinnatifida (2022) Marine Drugs. 10.3390/md20030197
Combining Lactate Oxidase and Metformin in Cancer Cell Membrane-Biomimetic Liposomes for Synergistic Ferroptosis Induction and Hypoxia-Alleviated Cancer Therapy (2026) COLLOIDS AND SURFACES B-BIOINTERFACES. 10.1016/j.colsurfb.2026.115420
Mertk-driven tunneling nanotube formation in macrophages preserves mitochondrial homeostasis during kidney Ischemia-Reperfusion Attack (2026) BIOCHEMICAL PHARMACOLOGY. 10.1016/j.bcp.2026.117713
Exosomal miR-127-5p from BMSCs alleviated sepsis-related acute lung injury by inhibiting neutrophil extracellular trap formation (2023) INTERNATIONAL IMMUNOPHARMACOLOGY. 10.1016/j.intimp.2023.110759
Anti-tumor effect of flavonoids isolated from Bidens Pilosa L. by regulating the activity of myeloid-derived suppressor cells within the tumor microenvironment in mice (2025) JOURNAL OF ETHNOPHARMACOLOGY. 10.1016/j.jep.2025.120635
