| Field | Specification |
|---|---|
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Applications | |
| Conjugate | |
| Concentration | |
| Storage | |
| Storage buffer | |
| Shipping | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
IFN-γ is a potent multifunctional cytokine which is secreted primarily by activated NK cells and T cells. Originally characterized based on anti-viral activities, IFN-γ also exerts anti-proliferative, immunoregulatory, and proinflammatory activities. IFN-γ can upregulate MHC class I and II antigen expression by antigen-presenting cells.
Validated Applications & Recommended Dilutions
| Application | Recommended Dilution |
|---|---|
| Flow Cytometry | See product datasheet |
| Immunophenotyping | See product datasheet |
Antibody Specifications
| Target | IFNγ |
|---|---|
| Host Species | Rat |
| Clonality | Monoclonal |
| Isotype | Rat IgG1, κ |
| Conjugate | PE |
| Clone Number | XMG1.2 |
| Purification | See datasheet |
| Concentration | 0.2 mg/mL |
Immunogen
Recombinant protein fragment; see full datasheet. UniProt: P01580.
Species Reactivity & Cross-Reactivity
Validated for: Mouse. Verify suitability in your sample type prior to use. For species or applications not listed in the datasheet, consult our technical team before purchase.
Storage & Handling
This product can be stored at 2-8°C for 12 months. Please protected from prolonged exposure to light and do not freeze.
Storage Buffer: Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Related Products
Browse additional antibodies targeting IFNγ and complementary reagents — including secondary antibodies, blocking buffers, and detection kits — available through BioHippo.
Yes — this antibody is validated for both surface and intracellular flow cytometry. For intracellular targets, fix and permeabilize cells using a validated fixation/permeabilization kit (e.g., BD Cytofix/Cytoperm or similar) before staining. For surface markers, stain live or fixed cells without permeabilization. Always include a matched isotype control (Rat IgG1, κ, Rat origin) at the same protein concentration as the test antibody to establish non-specific binding thresholds. Recommended starting dilution/concentration: see datasheet.
This antibody has been experimentally validated in: Mouse. Cross-reactivity with other species has not been systematically evaluated. If your sample species is not listed, assess immunogen sequence homology to your target species ortholog. The immunogen used is: "" — higher sequence identity between species increases the probability of cross-reactivity, but experimental validation with appropriate controls is necessary before drawing scientific conclusions.
This product can be stored at 2-8°C for 12 months. Please protected from prolonged exposure to light and do not freeze. Storage Buffer: Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.. To maintain antibody activity, avoid repeated freeze-thaw cycles. Aliquot into single-use volumes before first freeze. If working with the antibody frequently, keep a working stock at 4°C for up to 4 weeks — add 0.02% sodium azide to the working stock as a bacteriostatic agent if azide is not already present in the buffer. Monitor performance against a validated positive control when transitioning between storage aliquots or lots.
This monoclonal antibody (clone XMG1.2) provides highly consistent, epitope-specific recognition of IFNγ, making it ideal for quantitative assays, multiplex panels, and studies requiring strict reproducibility across experiments and independent laboratories. Unlike polyclonal preparations, each production batch from a single hybridoma clone generates antibody with identical epitope specificity, affinity, and isotype — ensuring reliable signal comparison in longitudinal studies or multi-site collaborations. When citing this antibody in publications, include the clone designation (clone XMG1.2) to enable third-party reproducibility verification.
Customization & Add-ons: Can't find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Harnessing Prazosin for Tumors: Liposome Hybrid Nanovesicles Activate Tumor Immunotherapy via Autophagy Inhibition (2025) BIOMATERIALS. 10.1016/j.biomaterials.2025.123184
Arginine-assembly as NO nano-donor prevents the negative feedback of macrophage repolarization by mitochondrial dysfunction for cancer immunotherapy (2024) BIOMATERIALS. 10.1016/j.biomaterials.2024.122474
Homologous magnetic targeted immune vesicles for amplifying immunotherapy via ferroptosis activation augmented photodynamic therapy against glioblastoma (2025) JOURNAL OF CONTROLLED RELEASE. 10.1016/j.jconrel.2025.113816
Endostatin induces normalization of blood vessels in colorectal cancer and promotes infiltration of CD8+ T cells to improve anti-PD-L1 immunotherapy (2022) Frontiers in Immunology. 10.3389/fimmu.2022.965492
Drug Repurposing with Topical Paclitaxel Delivery for Psoriasis Treatment Enabled by Plant-Inspired Molecular Assembly (2025) ACS Applied Materials & Interfaces. 10.1021/acsami.5c08843
The reduction effect on sensitization of sesame protein Ses i 3 of ultrasound-assisted glycation treatment through modulation of T cell differentiation (2025) INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES. 10.1016/j.ijbiomac.2025.142112
Rationally Designed Highly Potent NKT Cell Agonists with Different Cytokine Selectivity through Hydrogen-Bond Interaction (2024) JOURNAL OF MEDICINAL CHEMISTRY. 10.1021/acs.jmedchem.4c00782
Antidiabetic and Immunoregulatory Activities of Extract of Phyllanthus emblica L. in NOD with Spontaneous and Cyclophosphamide-Accelerated Diabetic Mice (2023) INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES. 10.3390/ijms24129922
Fuzheng Shengbai Decoction Enhances Antitumor Immunity via YTHDC2-Dependent Stabilization of CLCA2 mRNA in Colorectal Cancer (2026) JOURNAL OF ETHNOPHARMACOLOGY. 10.1016/j.jep.2026.121280
H5N1 influenza virus-like particles based on BEVS induce robust functional antibodies and immune responses (2025) VIROLOGY. 10.1016/j.virol.2025.110672
