PE/Cyanine7 Rat IgG2a, λ Isotype Control[B39-4]

SKU:BHA10327023
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    Overview
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    Anti-target protein antibody (Rat, Monoclonal, Rat IgG2a, λ, PE/Cyanine 7). Validated for Flow Cytometry in validated species samples. Suitable for detection and characterization of target protein in cell and tissue-based research.
    Clonality Monoclonal
    Host Species Rat
    Conjugate PE/Cyanine 7
    Isotype Rat IgG2a, λ
    Applications Flow Cytometry
    Clone B39-4
    Available Options

    Select the variant that best fits your experiment. Availability and lead time may vary by option.

    • Options: Size (3) - 20 Tests, 100 Tests, 100 Tests x 2
    • Lead time: options listed in “Availability Content”; other statuses may take longer.
    • Storage: This product can be stored at 2-8°C for 12 months. Please protected from prolonged exposure to light and do not freeze.
    • Shipping: cold-chain shipment (typically with ice packs).
    • Upon receipt: store at the recommended temperature as soon as possible.
    • Sales terms and conditions: Please review prior to ordering.
    Options selector
    Catalog no. Size
    E-AB-F09742H_20Tests 20 Tests
    E-AB-F09742H_100Tests 100 Tests
    E-AB-F09742H_100Tests×2 100 Tests x 2
    Field Specification
    Clonality
    • Monoclonal
    Concentration 5 μL/Test
    Conjugate
    • PE/Cyanine 7
    Gene ID
    Host Rat
    Isotype
    • Rat IgG2a
    • λ
    Product Type
    • Monoclonal Antibody
    Shipping Ice packs
    Storage This product can be stored at 2-8°C for 12 months. Please protected from prolonged exposure to light and do not freeze.
    UniProt #

    Product Overview

    Validated Applications & Recommended Dilutions

    Application Recommended Dilution
    Flow Cytometry See product datasheet

    Antibody Specifications

    Target the target protein
    Host Species Rat
    Clonality Monoclonal
    Isotype Rat IgG2a, λ
    Conjugate PE/Cyanine 7
    Clone Number B39-4
    Purification See datasheet
    Concentration 5 μL/Test

    Immunogen

    Recombinant protein fragment; see full datasheet. UniProt: .

    Species Reactivity & Cross-Reactivity

    Verify suitability in your sample type prior to use. For species or applications not listed in the datasheet, consult our technical team before purchase.

    Storage & Handling

    This product can be stored at 2-8°C for 12 months. Please protected from prolonged exposure to light and do not freeze.

    Storage Buffer: Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.

    Related Products

    Browse additional antibodies targeting the target protein and complementary reagents — including secondary antibodies, blocking buffers, and detection kits — available through BioHippo.

    Can this antibody be used for intracellular flow cytometry (ICFCM) as well as surface staining?

    This antibody is validated for flow cytometry applications. For intracellular targets, fix and permeabilize cells using a validated fixation/permeabilization kit (e.g., BD Cytofix/Cytoperm or similar) before staining. For surface markers, stain live or fixed cells without permeabilization. Always include a matched isotype control (Rat IgG2a, λ, Rat origin) at the same protein concentration as the test antibody to establish non-specific binding thresholds. Recommended starting dilution/concentration: see datasheet.

    What species is this the target antibody validated in, and will it work in my model organism?

    This antibody has been experimentally validated in: validated species. Cross-reactivity with other species has not been systematically evaluated. If your sample species is not listed, assess immunogen sequence homology to your target species ortholog. The immunogen used is: "" — higher sequence identity between species increases the probability of cross-reactivity, but experimental validation with appropriate controls is necessary before drawing scientific conclusions.

    What are the storage and stability requirements for this antibody?

    This product can be stored at 2-8°C for 12 months. Please protected from prolonged exposure to light and do not freeze. Storage Buffer: Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.. To maintain antibody activity, avoid repeated freeze-thaw cycles. Aliquot into single-use volumes before first freeze. If working with the antibody frequently, keep a working stock at 4°C for up to 4 weeks — add 0.02% sodium azide to the working stock as a bacteriostatic agent if azide is not already present in the buffer. Monitor performance against a validated positive control when transitioning between storage aliquots or lots.

    What are the advantages of using a monoclonal antibody for the target detection?

    This monoclonal antibody (clone B39-4) provides highly consistent, epitope-specific recognition of the target, making it ideal for quantitative assays, multiplex panels, and studies requiring strict reproducibility across experiments and independent laboratories. Unlike polyclonal preparations, each production batch from a single hybridoma clone generates antibody with identical epitope specificity, affinity, and isotype — ensuring reliable signal comparison in longitudinal studies or multi-site collaborations. When citing this antibody in publications, include the clone designation (clone B39-4) to enable third-party reproducibility verification.

    Customization & Add-ons: Can't find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.

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