PEGylated Liposomal Acridine Orange Dye

What it means: Acridine Orange (Ex 485 nm / Em 535 nm) — the fluorescent dye and how its signal is read.

Why it matters:

• The dye decides which filter set or plate reader settings you need.
• Matching wavelengths to your instrument gives the strongest signal.
• Dyes with different colors can be combined in one experiment if their spectra do not overlap.
• Running unlabeled controls helps you subtract background fluorescence.
• Stating the dye and wavelengths makes your methods easy to repeat.
• Controls with free dye help show whether a signal comes from the labeled material or from released dye.
• Photobleaching can lower the signal, so limit light exposure before reading.

For this product: The liposomes carry Acridine Orange (Ex 485 nm / Em 535 nm) at 0.1 mM.

Tip: Run empty liposomes or free dye as controls to interpret the signal.

What it means: HSPC : DSPE-PEG2000 : cholesterol = 3 : 1 : 1 — the lipids that form the bilayer.

Why it matters:

• Lipid composition affects membrane rigidity, stability and drug release.
• PEGylated lipids add a polymer layer that changes how liposomes interact with proteins and cells.
• Cholesterol makes the bilayer more stable and less leaky.
• Matching composition to your study makes results easier to compare with published work.
• Stating the composition makes your methods easy to report and repeat.
• The phase transition temperature of the main lipid affects how the bilayer behaves at assay temperature.
• Changing composition between experiments can change results even with the same cargo.

For this product: The bilayer is HSPC : DSPE-PEG2000 : cholesterol = 3 : 1 : 1 (w/w).

Tip: Choose the PEGylated or non-PEGylated version to match the liposomes you study.

What it means: About 80 nm — the average diameter of the liposomes.

Why it matters:

• Liposome size affects how liposomes move through tissues and how they are taken up by cells.
• Size also affects how fast encapsulated material is released.
• A narrow size range makes results more reproducible.
• Knowing the size helps you choose filters or columns for purification.
• Stating the size makes your methods easy to report and repeat.
• Size can be checked by dynamic light scattering before use.
• Comparing size before and after an experiment shows whether liposomes stayed intact.
• Particle size also influences how liposomes scatter light in optical measurements.

For this product: The liposomes average about 80 nm in diameter.

Tip: Measure size again after your experiment if stability matters.

What it means: Cold, Not Frozen — how the product is shipped.

Why it matters:

• The shipping condition tells you what to expect when the package arrives.
• Unpacking promptly lets you check the contents against the order.
• Checking the package on arrival helps you spot transport problems early.
• Storage conditions can differ from shipping conditions, so confirm them before the package arrives.
• Recording the arrival date and condition helps if questions come up later.
• Arranging for someone to receive the package avoids delays when it arrives.
• The shipping condition is chosen to keep the product as described during transport.

For this product: Ships cold, not frozen. Please contact us to confirm storage conditions.

Tip: Do not freeze liposomes; check the package on arrival and contact us right away if anything is damaged or missing.