| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Programmed cell death protein 1, Pdcd1, CD279 |
| Clonality | |
| Concentration | |
| Formulation | |
| Isotype | |
| Product Type | |
| Storage |
Product Overview
This is a research-grade recombinant antibody that reproduces the antigen-binding specificity of the reference therapeutic Pembrolizumab. Like the reference molecule, it binds PD-1 and blocks PD-L1/PD-L2 engagement, restoring T-cell response. It is intended for in vitro research use as a binding reagent, functional (blocking/neutralizing) tool, and as a positive control or reference standard in bioanalytical assays.
This product carries the same variable-region specificity as Pembrolizumab and therefore targets PD-1 (CD279). It is a research reagent and is not the licensed therapeutic product.
Target & Mechanism
Target antigen: PD-1 (CD279). The molecule binds PD-1 and blocks PD-L1/PD-L2 engagement, restoring T-cell response.
Research Applications
| Application | Notes |
|---|---|
| ELISA (antigen binding) | Use as a binding reagent against PD-1 (CD279); suitable as a capture/detection antibody and as an assay control. |
| Ligand blocking / neutralization | Functional blocking of PD-1 (CD279) in biochemical or cell-based assays. |
| Biophysics (SPR / BLI) | Kinetic and affinity characterization of binding to PD-1 (CD279). |
| Flow cytometry | Detection of cell-surface PD-1 (CD279) on relevant cell types. |
| PK / ADA assay control | Positive control or reference standard in pharmacokinetic (drug-level) and anti-drug-antibody (immunogenicity) assays for Pembrolizumab. |
Specifications
| Reference product | Pembrolizumab |
|---|---|
| Molecular format | Humanized monoclonal antibody |
| Target antigen | PD-1 (CD279) |
| Isotype / class | IgG4κ |
| Expression | Recombinant, mammalian cell expression (see datasheet for lot details) |
| Purity / concentration / endotoxin | Lot-specific — stated on the Certificate of Analysis |
| Formulation | Supplied in PBS, pH 7.0 (see datasheet) |
| Storage | -20°C long term; avoid repeated freeze–thaw cycles |
Validation & QC
Certificate of Analysis supplied per lot
Typical release testing includes identity/purity by SDS-PAGE and confirmation of PD-1 (CD279) binding by ELISA or SPR/BLI. Full validation data and the product datasheet are available on request.
Safety & Handling
For Research Use Only (RUO). Not for diagnostic, therapeutic, or human/animal in vivo use. Handle in accordance with good laboratory practice.
Related Products
Pair with the matching anti-idiotypic antibody and the Pembrolizumab drug-level (PK) and anti-drug-antibody (ADA) ELISA kits for a complete bioanalytical workflow. Explore related antibodies, kits and reagents at BioHippo.
This antibody has been validated for multiple applications. Always refer to the product datasheet for recommended dilution ranges and appropriate positive control samples for each application type.
Validated reactivity has been confirmed for Human. Cross-reactivity with additional species may exist based on sequence homology but has not been confirmed by the manufacturer. For unlisted species, we recommend running a preliminary validation experiment.
This antibody was raised in Human. For indirect detection (WB, IHC, IF), select an anti-Human secondary antibody that matches the isotype. For direct detection, check the variant selector for conjugated formats. Confirm secondary antibody compatibility before ordering.
Please refer to the product datasheet for detailed immunogen information. This data is available on the manufacturer datasheet linked in the Documents tab.
Please refer to the storage conditions on the product label and datasheet. As a general best practice: upon receipt, aliquot into single-use volumes to minimize freeze-thaw cycling, which can progressively reduce antibody performance and lead to aggregation.
Validated reactivity covers Human samples. Performance can vary across different cell lines and tissue types depending on target antigen expression levels and epitope accessibility. We recommend verifying target expression in your specific model system using a known positive control before proceeding to experimental samples.
BioHippo offers customization and value-added services for qualified research programs:
- Bulk Pricing: Volume discounts available for orders of 5 or more units. Request a quote through the Contact Us page.
- Custom Conjugation: Select antibody products are available for custom conjugation to fluorophores, biotin, HRP, or other detection labels upon request. Minimum quantities apply.
- Custom Formats: Lyophilized or concentrated formats may be available for select products. Inquire before ordering.
- Technical Support: Our scientific team is available to assist with application optimization, protocol troubleshooting, and experimental design.
For customization inquiries, please use the Contact Us page or reach out to our support team directly.
