PEPA

Product details

• Chemical name: 2-(2,6-difluoro-4-(2-(phenylsulfonamido)ethylthio)phenoxy)acetamide.
• CAS number: 141286-78-4
• Molecular formula: C16H16F2N2O4S2.
• Purity: >99.9%
• Concentration: 10 - 250 μM.
• Form: Lyophilized powder.
• Solubility: 50 mM in DMSO. Centrifuge all product preparations before use (10000 x g for 1 min).
• Reconstitution: Centrifuge the vial before adding solvent (10,000 x g for 5 minutes) to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity
• Shipping: Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
• Target: AMPA receptors
• Source: Synthetic
• Activity: PEPA is a selective AMPA receptor agonist (allosteric potentiator)1.

Scientific background

PEPA is a flop splice variant and GluR3/4-prefrerring potentiator of AMPA receptors. PEPA is small chemical compound that enhances AMPA receptor channel activity by modulating receptor kinetics such as desensitization and deactivation. It reportedly enhances synaptic response by selectively acting on AMPA receptors. PEPA (1-200 μM) dose dependently potentiates glutamate-evoked currents in Xenopus oocytes expressing AMPA (GluRA-GluRD). At 200 μM, PEPA could potentiate glutamate responses by 50-fold in oocytes expressing GluRCflop (EC50 ∼50 μM)1. In addition, it potently facilitates extinction learning through activation of AMPA receptors mainly in the medial prefrontal cortex (mPFC)2.

Lead time: 1-2 Business Days

Country of origin: Israel/IL

Applications key

Application key: FC- Flow cytometry, IFC- Indirect flow cytometry, IHC- Immunohistochemistry,LCI- Live cell imaging, Calcium imaging assay,Cell survival assay, Electrophysiology, Neurite outgrowth assay.

Bioassay tested: Yes

• SDS (PDF)

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• Add-on labels & handles: D/¹³C/¹⁵N isotopes (LC-MS/internal standards), azide/alkyne or other functional handles for conjugation
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