| Field | Specification |
|---|---|
| Target | |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A portion of amino acids 266-296 from the human protein was used as the immunogen for the PFKFB4 antibody. |
| Purity | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Overview
PFKFB4 Antibody / PFK/FBPase 4 is an antibody targeting PFKFB4, raised in Rabbit for protein detection and localization studies where these specifications are required.
Key elements and design rationale
- Target: PFKFB4 (reported localization: Cytoplasm).
- Antibody identity: Polyclonal (rabbit origin); Rabbit Ig.
- Conjugate/label: Unconjugated (affects detection chemistry and multiplex compatibility).
- Format: Purified.
- Species reactivity: Human.
- Listed applications: WB, IHC-P (refer to on-page specifications for application-specific guidance).
Biological background
PFKFB4, also called 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 4, is a key enzyme involved in the regulation of cellular metabolism. It acts as a regulator of the glycolysis process, which is the breakdown of glucose into energy. This enzyme is crucial for maintaining the balance between energy production and storage within the cell. Research has shown that dysregulation of PFKFB4 can have profound effects on cellular metabolism and ultimately, overall health. For example, overexpression of PFKFB4 has been linked to increased glycolysis and cancer cell proliferation, making it a potential target for cancer therapy. On the other hand, decreased levels of PFKFB4 have been associated with metabolic disorders such as diabetes. One particularly intriguing aspect of PFKFB4 is its dual function as both a kinase and a phosphatase. This unique feature allows it to not only stimulate glycolysis but also inhibit the breakdown of fructose-2,6-biphosphate, a key activator of glycolysis. This dual role gives PFKFB4 a central position in the complex regulatory network of cellular metabolism.
Research relevance and current trends
- Comparative expression profiling across cell types, tissues, or perturbations (e.g., drug treatment, genetic editing, or differentiation).
- Subcellular localization and trafficking studies, including co-localization with pathway markers in microscopy-based assays.
- Integration of protein-level measurements with transcriptomics or proteomics to relate abundance to regulation and phenotype.
Common research applications
- Western blotting: researchers commonly compare relative signal levels across conditions and use appropriate negative/positive controls for interpretation.
- Immunohistochemistry: researchers commonly compare relative signal levels across conditions and use appropriate negative/positive controls for interpretation.
Interpretation should account for antibody-dependent factors such as epitope accessibility, isoforms, and sample preparation differences across workflows.
Notes for experimental interpretation
- Isoforms and PTMs: many targets have multiple isoforms and post-translational modifications that can shift apparent signal or localization; interpret bands/signals accordingly.
- Epitope context: binding can depend on protein conformation and sample processing; region information in the title/immunogen can help anticipate what may be detected.
- Species differences: predicted or validated reactivity may vary by ortholog sequence and sample context; confirm in your model system.
- Control concepts: include negative controls (no-primary/isotype), and where possible genetic controls (KO/KD) or independent antibodies to strengthen conclusions.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
