| Field | Specification |
|---|---|
| Product Type | |
| Shipping | |
| Source | Recombinant (E. coli) |
| Storage |
phi29 DNA polymerase is the replicative polymerase from Bacillus subtilis bacteriophage, has exceptional strand displacement and processive synthesis properties. It can synthesize DNA fragments up to 70 kb. The polymerase has an inherent 3´→5' proofreading exonuclease activity. And it is recommended to modify the 3 'end of the primer in the system to prevent degradation. phi29 DNA polymerase is often used for plasmid synthesis in vitro and whole genome synthesis.
Features
High sensitivity: It is capable of effectively amplifying DNA templates at the pg level.
High amplification yield: When 100 ng of template is input, the yield can reach 2 μg/μL.
Applications
DNA Enzymatic Synthesis.
Whole genome amplification (WGA).
Rolling circle amplification (RCA).
Multiplex displacement amplification (MDA).
SNP detection.
Specifications
|
Unit Definition |
One unit is defined as the amount of enzyme that will incorporate 0.5 pmol of dNTP into acid insoluble material in 10 minutes at 30°C. |
|
Heat Inactivation |
65°C for 10 min |
Glycerol Content |
Contains Glycerol |
Components
|
Components No. |
Name |
14404ES72 |
14404ES80 |
14404ES90 |
14404ES92 |
|
14404-A |
phi29 DNA Polymerase (10 U/μL） |
25 μL |
100 μL |
500 μL |
1 mL |
|
14404-B |
10×phi29 Reaction Buffer |
100 μL |
400 μL |
2×1 mL |
4×1 mL |
Shipping and Storage
This product should be stored at -25 ~ -15oC for 2 years.
Figures
Figure 1.Whole genome amplification with different DNA template input amounts using phi29 DNA polymerases from Yeasen and Supplier A . C1: No template control, C2: No enzyme control. The results showed that phi29 DNA polymerase (Cat#14404) had better sensitivity and amplification yield than the competitor's product.
Figure 2.The amplification yield of Yeasen phi29 DNA polymerase. Template: 293 cell gDNA, with an input of 100 ng; Reaction time: 16 h. The results showed that the amplification yield of Yeasen phi29 DNA polymerase (Cat#14404) could reach 2 μg/μL when the input amount of template was 100 ng.
Store this enzyme at -20°C and avoid repeated freeze-thaw cycles to preserve catalytic activity. The product is shipped Dry Ice and remains stable for up to one year from the date of manufacture when stored under recommended conditions. Aliquoting the stock solution into single-use volumes is recommended for enzymes used infrequently to minimize thermal cycling of the bulk stock.
DNA Enzymatic Synthesis. Whole genome amplification (WGA). Rolling circle amplification (RCA). Multiplex displacement amplification (MDA). SNP detection.. Always verify compatibility with your specific template, buffer, and downstream workflow.
One unit (U) is defined as the amount of enzyme that catalyzes the incorporation of 10 nmol of deoxyribonucleotide into acid-insoluble material in 30 min under standard assay conditions.
This enzyme is produced as Recombinant (E. coli) and supplied as a Research Use Only (RUO) reagent. Each lot is subjected to activity assay, purity assessment by SDS-PAGE, and functional validation prior to release. A Certificate of Analysis (CoA) and Safety Data Sheet (SDS) are available on request.
This enzyme is produced recombinantly in E. coli under stringent quality controls and validated on multiple substrate types. Key performance parameters include specific activity, substrate specificity, cofactor requirements, optimal pH/temperature range, and compatibility with downstream assays. Refer to the product datasheet for validated lot-specific activity data and recommended reaction conditions.
Yeasen Biotechnology supports custom enzyme solutions across multiple service lines — from GMP-grade bulk supply to directed enzyme engineering. Contact BioHippo to discuss requirements and initiate a project inquiry.
▶ GMP-Grade & Bulk Supply
Select Yeasen enzymes are available in GMP grade, manufactured in an ISO 13485-certified UCF.ME™ ultra-clean molecular enzyme facility with FDA Drug Master File (DMF) support.
- GMP-grade release testing and CoA documentation
- ISO 13485-certified production facility
- Scalable from milligram to multi-gram quantities
- Consistent lot-to-lot activity specifications
▶ Glycerol-Free & Custom Formulation
Glycerol-free enzyme formats are available for applications requiring lyophilization compatibility, liquid handling automation, or direct IVD master mix integration.
- Glycerol-free liquid format (standard and custom buffers)
- Lyophilization-ready enzyme preparation
- Custom reaction buffer optimization for specific assay conditions
- Compatible with freeze-drying workflows for point-of-care formats
▶ Molecular IVD RDC Service
Yeasen's Research and Development Contracting (RDC) team delivers end-to-end solutions for molecular diagnostic product development, covering enzyme selection through clinical validation support.
- Enzyme selection and performance matching
- Primer/probe design and reaction buffer optimization
- Sensitivity, specificity, and precision validation studies
- Stability studies and SNP evaluation
- Instrument platform compatibility assessment
▶ ZymeEditor™ Enzyme Engineering
Yeasen's proprietary ZymeEditor™ directed evolution and rational design platform enables the development of custom enzyme variants with tailored performance characteristics not available in off-the-shelf products.
- Directed evolution for enhanced thermostability, processivity, or fidelity
- Rational design for altered substrate specificity or cofactor requirements
- Library screening from Yeasen's proprietary enzyme variant collection
- Scale-up to commercial quantities upon candidate confirmation
ⓘ Customization services are fulfilled by Yeasen Biotechnology. Lead times and minimum order quantities vary by service type. Contact BioHippo for project scoping and pricing.
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