Phospho-AKT1 (T72) Antibody

SKU:BHA10538702
Overview
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AKT1-specific polyclonal antibody raised in Rabbit. Validated for Western Blot, Immunohistochemistry, ELISA in Human, Mouse, Rat samples. Affinity-purified.
Format Unconjugated
Immunogen Synthesized peptide derived from Human Akt1 around the phosphorylation site of T72.
Applications 3 validated
Dilution WB:1:500-1:2000, IHC:1:100-1:300, ELISA:
Target Aliases 22 aliases
Available Options
Size
Options selector
Catalog no. Size
CSB-PA008120-50UG 50 ug
CSB-PA008120-100UG 100 ug
Field Specification
Alternative Names AKT 1; AKT; AKT1; AKT1_HUMAN; C AKT; cAKT; MGC99656; PKB alpha; PKB; PKB-ALPHA; PRKBA; Protein Kinase B Alpha; Protein kinase B; Proto-oncogene c-Akt; RAC Alpha; RAC; Rac protein kinase alpha; RAC Serine/Threonine Protein Kinase; RAC-alpha serine/threonine-protein kinase; RAC-PK-alpha; v akt murine thymoma viral oncogene homolog 1; vAKT Murine Thymoma Viral Oncogene Homolog 1
Antigen
  • Synthesized peptide derived from Human Akt1 around the phosphorylation site of T72.
Clonality
  • Polyclonal
Conjugate
  • Non-conjugated
Form Liquid
Host Rabbit
Immunogen Synthesized peptide derived from Human Akt1 around the phosphorylation site of T72.
Isotype
  • IgG
Product Type
  • Primary Antibody
Reactivity
  • Human
  • Mouse
  • Rat
Source This product is a polyclonal antibody purified from rabbit antiserum.
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target AKT1
UniProt # P31749

Product Overview

Primary Antibody

This antibody targets AKT1 and was raised against a recombinant protein immunogen.

Validated Applications

Application Recommended Dilution
Western Blot (WB) WB:1:500-1:2000, IHC:1:100-1:300, ELISA:1:5000
Immunohistochemistry (IHC) WB:1:500-1:2000, IHC:1:100-1:300, ELISA:1:5000
ELISA WB:1:500-1:2000, IHC:1:100-1:300, ELISA:1:5000
✔ Validated by CUSABIO Technology

Antibody Specifications

Target AKT1
Host Rabbit
Clonality Polyclonal
Isotype IgG
Conjugate Unconjugated
Reactivity Human, Mouse, Rat
Purification Affinity Purification
Antigen / Immunogen Synthesized peptide derived from Human Akt1 around the phosphorylation site of T72.
Production This product is a polyclonal antibody purified from rabbit antiserum.
Form Liquid
Storage Buffer PBS, 50% glycerol, 0.5% BSA, 0.02% sodium azide, pH 7.4.
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Alternative Names

AKT 1; AKT; AKT1; AKT1_HUMAN; C AKT; cAKT; MGC99656; PKB alpha; PKB; PKB-ALPHA; PRKBA; Protein Kinase B Alpha; Protein kinase B; Proto-oncogene c-Akt; RAC Alpha; RAC; Rac protein kinase alpha; RAC Serine/Threonine Protein Kinase; RAC-alpha serine/threonine-protein kinase; RAC-PK-alpha; v akt murine thymoma viral oncogene homolog 1; vAKT Murine Thymoma Viral Oncogene Homolog 1

Safety & Handling

⚠ Research Use Only (RUO). Not for diagnostic or therapeutic use. Handle with standard laboratory precautions. Dispose per local regulations.
TargetAKT1
HostRabbit
ClonalityPolyclonal
IsotypeIgG
ConjugateUnconjugated
ReactivityHuman, Mouse, Rat
PurificationAffinity Purification
Antigen/ImmunogenSynthesized peptide derived from Human Akt1 around the phosphorylation site of T72.
ProductionThis product is a polyclonal antibody purified from rabbit antiserum.
FormLiquid
Storage BufferPBS, 50% glycerol, 0.5% BSA, 0.02% sodium azide, pH 7.4.
StorageUpon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Dilutions are starting recommendations. Titration is advised for each system.

ApplicationRecommended Dilution
Western Blot (WB)WB:1:500-1:2000, IHC:1:100-1:300, ELISA:1:5000
Immunohistochemistry (IHC)WB:1:500-1:2000, IHC:1:100-1:300, ELISA:1:5000
ELISAWB:1:500-1:2000, IHC:1:100-1:300, ELISA:1:5000

Immunogen: Synthesized peptide derived from Human Akt1 around the phosphorylation site of T72.

Antigen Species: Human

Alternative Names

AKT 1; AKT; AKT1; AKT1_HUMAN; C AKT; cAKT; MGC99656; PKB alpha; PKB; PKB-ALPHA; PRKBA; Protein Kinase B Alpha; Protein kinase B; Proto-oncogene c-Akt; RAC Alpha; RAC; Rac protein kinase alpha; RAC Serine/Threonine Protein Kinase; RAC-alpha serine/threonine-protein kinase; RAC-PK-alpha; v akt murine thymoma viral oncogene homolog 1; vAKT Murine Thymoma Viral Oncogene Homolog 1

What species does this AKT1 antibody react with?
This antibody has been validated for reactivity against Human, Mouse, Rat. Cross-reactivity is based on the immunogen sequence and may vary by sample type.
What applications has this antibody been validated for?
Validated applications include: Western Blot (WB), Immunohistochemistry (IHC), ELISA. Each application was confirmed with appropriate controls. Contact technical support for applications not listed.
What is the recommended dilution?
WB:1:500-1:2000, IHC:1:100-1:300, ELISA:1:5000. Optimal concentrations depend on sample type, detection system, and antigen abundance. Titration with positive and negative controls is recommended.
How was this antibody produced?
This polyclonal IgG antibody was raised in Rabbit and purified by affinity chromatography using Protein G. It provides high specificity and minimal cross-reactivity in standard immunoassay applications.
What are the storage requirements?
Store at −20°C for long-term. Avoid repeated freeze-thaw cycles. Short-term working aliquots may be stored at 4°C. This product is supplied in a stabilized buffer containing BSA and sodium azide.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.

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