Phospho-AR (Ser213) Antibody

Overview

This is a polyclonal anti-AR antibody raised in Rabbit, with confirmed utility in ELISA, WB, IF. It is designed to detect AR protein in Human and supports researchers working in signal transduction contexts.

Key elements and design rationale

• Immunogen: Peptide sequence around phosphorylation site of serine 213 (E-A-S(p)-G-A) derived from Human Androgen Receptor. — determines the epitope region; confirm compatibility with sample preparation and expected post-translational modifications.
• Host species (Rabbit): Requires anti-rabbit-IgG secondary reagents for indirect detection.
• Polyclonal format: Recognizes multiple epitopes for robust signal; inherent lot-to-lot variability requires appropriate controls.
• Isotype (IgG): Matched secondary antibodies and isotype controls required. Compatible with standard Protein A/G purification workflows.
• Purification (Antigen affinity purification): Enriches for specific immunoglobulin classes; reduces non-specific background vs. crude antisera.

Biological background

AR (also referred to as AIS, ANDR_HUMAN, Androgen nuclear receptor variant 2, Androgen receptor (dihydrotestosterone receptor, testicular feminization, spinal and bulbar muscular atrophy, Kennedy disease), Androgen receptor, androgen receptor splice variant 4b, AR, AR8, DHTR, Dihydro testosterone receptor, Dihydrotestosterone receptor (DHTR), Dihydrotestosterone receptor, HUMARA, HYSP1, KD, Kennedy disease (KD), NR3C4, Nuclear receptor subfamily 3 group C member 4 (NR3C4), Nuclear receptor subfamily 3 group C member 4, SBMA, SMAX1, Spinal and bulbar muscular atrophy (SBMA), Spinal and bulbar muscular atrophy, Testicular Feminization (TFM), TFM) is a protein target studied in Human systems. Expression, subcellular localization, and post-translational modifications vary across cell types and disease states — factors critical to antibody-based detection design. Consult UniProt, NCBI Gene, and primary literature for current annotation of AR biology in signal transduction.

Common research applications

• ELISA: Quantification of soluble target in biological fluids or culture supernatants. Ensure samples are within the linear detection range.
• WB: Confirms target molecular weight and distinguishes isoforms under denaturing conditions.
• IF: Subcellular localization in fixed cells or tissue sections; fixation and permeabilization conditions affect epitope accessibility.

Notes for experimental interpretation

• Isotype controls: Use an isotype-matched (IgG from Rabbit) control at equivalent concentration to assess non-specific background.
• Cross-reactivity: Polyclonal preparations may cross-react with related proteins. Orthogonal validation (siRNA, KO lysate) is recommended.
• Matrix effects: Sample matrix can affect performance; pilot dilution linearity and spike-recovery experiments are advised for quantitative studies.
• Species reactivity: Confirmed for Human. Extrapolation to untested species requires empirical validation.

This product is a polyclonal antibody purified from rabbit antiserum.

• Datasheet (11082689)

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.