| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | BLK; Tyrosine-protein kinase Blk; B lymphocyte kinase; p55-Blk |
| Clonality | |
| Concentration | |
| Formulation | |
| Gene ID | |
| Host | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human BLK around the phosphorylation site of Tyr501. AA range:456-505 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Storage | |
| Target |
Product Overview
Primary AntibodyThis antibody targets BLK and detects endogenous levels of BLK protein. Only detects the phosphorylated form of BLK.
Validated Applications
| Application | Dilution Guidance |
|---|---|
| Immunohistochemistry (IHC) | Refer to datasheet for recommended working dilution |
| ELISA | Refer to datasheet for recommended working dilution |
Antibody Specifications
| Target | BLK |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Reactivity | Human, Mouse, Rat |
| Purification | Affinity Purification |
| Molecular Weight | ~57.7 kDa |
| Concentration | 1 mg/ml |
| Immunogen | The antiserum was produced against synthesized peptide derived from human BLK around the phosphorylation site of Tyr501. AA range:456-505 |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Storage | -20°C for 1 year |
| Gene ID | Human: 640; Mouse: 12143 |
| UniProt | P51451 (Human: P51451; Mouse: P16277) |
Scientific Background
BLK (BLK proto-oncogene, Src family tyrosine kinase) encodes a nonreceptor tyrosine-kinase of the src family of proto-oncogenes that are typically involved in cell proliferation and differentiation. The protein has a role in B-cell receptor signaling and B-cell development. The protein also stimulates insulin synthesis and secretion in response to glucose and enhances the expression of several pancreatic beta-cell transcription factors.
Alternative Names
BLK; Tyrosine-protein kinase Blk; B lymphocyte kinase; p55-Blk
Safety & Handling
| Target | BLK |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Reactivity | Human, Mouse, Rat |
| Purification | Affinity Purification |
| Molecular Weight | ~57.7 kDa |
| Concentration | 1 mg/ml |
| Immunogen | The antiserum was produced against synthesized peptide derived from human BLK around the phosphorylation site of Tyr501. AA range:456-505 |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Storage | -20°C for 1 year |
| Gene ID | Human: 640; Mouse: 12143 |
| UniProt | P51451 (Human: P51451; Mouse: P16277) |
Optimized working dilutions depend on sample type and detection system. Titration is recommended.
| Application | Dilution Note |
|---|---|
| Immunohistochemistry (IHC) | Refer to datasheet for recommended working dilution |
| ELISA | Refer to datasheet for recommended working dilution |
BLK (BLK proto-oncogene, Src family tyrosine kinase) encodes a nonreceptor tyrosine-kinase of the src family of proto-oncogenes that are typically involved in cell proliferation and differentiation. The protein has a role in B-cell receptor signaling and B-cell development. The protein also stimulates insulin synthesis and secretion in response to glucose and enhances the expression of several pancreatic beta-cell transcription factors.
Alternative Names
BLK; Tyrosine-protein kinase Blk; B lymphocyte kinase; p55-Blk
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
