Phospho-CaMKIV (T200) Polyclonal Antibody

SKU:BHA12105080
Overview
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CAMK4-specific polyclonal antibody raised in Rabbit. Validated for Western Blot, Immunohistochemistry, Immunofluorescence in Human, Mouse, Rat samples. Phospho-specific. Affinity-purified rabbit IgG.
Specificity Phospho-Specific
Immunogen Synthetic Peptide
Molecular Weight ~51.9 kDa
UniProt Q16566
Gene ID Human: 814; Rat: 25050
Applications 4 validated
Options selector
Catalog no. Size
BT-PHS00043-20UL 20 uL
BT-PHS00043-50UL 50 uL
BT-PHS00043-100UL 100 uL
Available Options
Size
Field Specification
Mfr No BT-PHS00043
Alternative Names CAMK4; CAMK; CAMK-GR; CAMKIV; Calcium/calmodulin-dependent protein kinase type IV; CaMK IV; CaM kinase-GR
Clonality
  • Polyclonal
Concentration 1 mg/ml
Formulation 1 mg/ml, liquid in 0.01M Phosphate Buffered Saline, pH 7.2, containing 1% BSA, 50% glycerol, 0.02% Sodium Azide
Gene ID Human: 814; Rat: 25050
Host Rabbit
Immunogen The antiserum was produced against synthesized peptide derived from human CaMK4 around the phosphorylation site of Thr196/200. AA range:166-215
Isotype
  • IgG
Molecular Weight ~51.9 kDa
Product Type
  • Primary Antibody
Reactivity
  • Human
  • Mouse
  • Rat
Storage -20°C for 1 year
Target CAMK4

Product Overview

Primary Antibody

This antibody targets CAMK4 and detects endogenous levels of CAMK4 protein. Only detects the phosphorylated form of CAMK4.

Validated Applications

Application Dilution Guidance
Western Blot (WB) Refer to datasheet for recommended working dilution
Immunohistochemistry (IHC) Refer to datasheet for recommended working dilution
Immunofluorescence (IF) Refer to datasheet for recommended working dilution
ELISA Refer to datasheet for recommended working dilution
✔ Validated by Bioassay Technology Laboratory

Antibody Specifications

Target CAMK4
Host Rabbit
Clonality Polyclonal
Isotype IgG
Reactivity Human, Mouse, Rat
Purification Affinity Purification
Molecular Weight ~51.9 kDa
Concentration 1 mg/ml
Immunogen The antiserum was produced against synthesized peptide derived from human CaMK4 around the phosphorylation site of Thr196/200. AA range:166-215
Formulation 1 mg/ml, liquid in 0.01M Phosphate Buffered Saline, pH 7.2, containing 1% BSA, 50% glycerol, 0.02% Sodium Azide
Storage -20°C for 1 year
Gene ID Human: 814; Rat: 25050
UniProt Q16566 (Human: Q16566; Mouse: P08414; Rat: P13234)

Scientific Background

The product of CAMK4 (calcium/calmodulin dependent protein kinase IV) belongs to the serine/threonine protein kinase family, and to the Ca(2+)/calmodulin-dependent protein kinase subfamily. This enzyme is a multifunctional serine/threonine protein kinase with limited tissue distribution, that has been implicated in transcriptional regulation in lymphocytes, neurons and male germ cells.

Alternative Names

CAMK4; CAMK; CAMK-GR; CAMKIV; Calcium/calmodulin-dependent protein kinase type IV; CaMK IV; CaM kinase-GR

Safety & Handling

⚠ Research Use Only (RUO). Not approved for diagnostic or therapeutic use. Handle with standard laboratory safety precautions. Contains sodium azide — avoid contact with skin/eyes; dispose per local regulations.
TargetCAMK4
HostRabbit
ClonalityPolyclonal
IsotypeIgG
ReactivityHuman, Mouse, Rat
PurificationAffinity Purification
Molecular Weight~51.9 kDa
Concentration1 mg/ml
ImmunogenThe antiserum was produced against synthesized peptide derived from human CaMK4 around the phosphorylation site of Thr196/200. AA range:166-215
Formulation1 mg/ml, liquid in 0.01M Phosphate Buffered Saline, pH 7.2, containing 1% BSA, 50% glycerol, 0.02% Sodium Azide
Storage-20°C for 1 year
Gene IDHuman: 814; Rat: 25050
UniProtQ16566 (Human: Q16566; Mouse: P08414; Rat: P13234)

Optimized working dilutions depend on sample type and detection system. Titration is recommended.

ApplicationDilution Note
Western Blot (WB)Refer to datasheet for recommended working dilution
Immunohistochemistry (IHC)Refer to datasheet for recommended working dilution
Immunofluorescence (IF)Refer to datasheet for recommended working dilution
ELISARefer to datasheet for recommended working dilution

The product of CAMK4 (calcium/calmodulin dependent protein kinase IV) belongs to the serine/threonine protein kinase family, and to the Ca(2+)/calmodulin-dependent protein kinase subfamily. This enzyme is a multifunctional serine/threonine protein kinase with limited tissue distribution, that has been implicated in transcriptional regulation in lymphocytes, neurons and male germ cells.

Alternative Names

CAMK4; CAMK; CAMK-GR; CAMKIV; Calcium/calmodulin-dependent protein kinase type IV; CaMK IV; CaM kinase-GR

What species does this antibody react with?
This antibody has been validated for reactivity against Human, Mouse, Rat. Actual cross-reactivity is determined by immunogen sequence homology across species. Contact technical support for unlisted species.
What applications has this antibody been validated for?
Validated applications include: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA. Each application was tested with positive and negative controls. Contact our team regarding applications not listed.
How was this antibody produced and purified?
This polyclonal IgG antibody was raised in rabbit against a synthesized target-specific peptide. It was affinity-purified from antiserum using an epitope-specific immunogen, yielding a highly specific antibody suitable for multiple research applications.
What is the expected molecular weight of CAMK4?
The predicted molecular weight of CAMK4 is ~51.9 kDa. Band size may vary slightly depending on post-translational modifications and gel percentage.
What storage conditions does this antibody require?
Store at −20°C for long-term storage. Avoid repeated freeze-thaw cycles. Working aliquots can be stored at 4°C for short-term use. The formulation includes 50% glycerol as a cryoprotectant.
Is this antibody specific for the phosphorylated form of CAMK4?
Yes. This antibody is designed to detect CAMK4 only at the phosphorylation site specified in the product name. Lambda phosphatase treatment eliminates the signal, confirming phospho-specificity.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.

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