Phospho-CD40 (T254) Antibody

SKU:BHA10539215
Overview
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CD40-specific polyclonal antibody raised in Rabbit. Validated for Western Blot, ELISA in Human, Mouse samples. Affinity-purified.
Format Unconjugated
Immunogen Synthesized peptide derived from Human CD40 around the phosphorylation site of T254.
Applications 2 validated
Dilution WB:1:500-1:2000, ELISA:1:5000
Target Aliases 35 aliases
Available Options
Size
Options selector
Catalog no. Size
CSB-PA080031-50UG 50 ug
CSB-PA080031-100UG 100 ug
Field Specification
Alternative Names AI326936; B cell associated molecule CD40; B cell surface antigen CD40; B cell-associated molecule; B-cell surface antigen CD40; Bp50; CD 40; CD40; CD40 antigen (TNF receptor superfamily member 5); CD40 antigen; CD40 molecule; CD40 molecule, TNF receptor superfamily member 5; CD40 protein; CD40 type II isoform; CD40L receptor; CDw40; GP39; HIGM1; IGM; IMD3; MGC9013; Nerve growth factor receptor related B lymphocyte activation molecule; OTTHUMP00000031699; OTTHUMP00000031700; p50; T-BAM; TBAM; TNF receptor superfamily member 5; TNFRSF5; TNR5_HUMAN; TRAP; Tumor necrosis factor receptor superfamily , member 5; Tumor necrosis factor receptor superfamily member 5; Tumor necrosis factor receptor superfamily member 5 precursor; Tumor necrosis factor receptor superfamily, member 5, isoform CRA_a
Antigen
  • Synthesized peptide derived from Human CD40 around the phosphorylation site of T254.
Clonality
  • Polyclonal
Conjugate
  • Non-conjugated
Form Liquid
Host Rabbit
Immunogen Synthesized peptide derived from Human CD40 around the phosphorylation site of T254.
Isotype
  • IgG
Product Type
  • Primary Antibody
Reactivity
  • Human
  • Mouse
Source This product is a polyclonal antibody purified from rabbit antiserum.
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target CD40
UniProt # P25942

Product Overview

Primary Antibody

This antibody targets CD40 and was raised against a recombinant protein immunogen.

Validated Applications

Application Recommended Dilution
Western Blot (WB) WB:1:500-1:2000, ELISA:1:5000
ELISA WB:1:500-1:2000, ELISA:1:5000
✔ Validated by CUSABIO Technology

Antibody Specifications

Target CD40
Host Rabbit
Clonality Polyclonal
Isotype IgG
Conjugate Unconjugated
Reactivity Human, Mouse
Purification Affinity Purification
Antigen / Immunogen Synthesized peptide derived from Human CD40 around the phosphorylation site of T254.
Production This product is a polyclonal antibody purified from rabbit antiserum.
Form Liquid
Storage Buffer PBS, 50% glycerol, 0.5% BSA, 0.02% sodium azide, pH 7.4.
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Alternative Names

AI326936; B cell associated molecule CD40; B cell surface antigen CD40; B cell-associated molecule; B-cell surface antigen CD40; Bp50; CD 40; CD40; CD40 antigen (TNF receptor superfamily member 5); CD40 antigen; CD40 molecule; CD40 molecule, TNF receptor superfamily member 5; CD40 protein; CD40 type II isoform; CD40L receptor; CDw40; GP39; HIGM1; IGM; IMD3; MGC9013; Nerve growth factor receptor related B lymphocyte activation molecule; OTTHUMP00000031699; OTTHUMP00000031700; p50; T-BAM; TBAM; TNF receptor superfamily member 5; TNFRSF5; TNR5_HUMAN; TRAP; Tumor necrosis factor receptor superfamily , member 5; Tumor necrosis factor receptor superfamily member 5; Tumor necrosis factor receptor superfamily member 5 precursor; Tumor necrosis factor receptor superfamily, member 5, isoform CRA_a

Safety & Handling

⚠ Research Use Only (RUO). Not for diagnostic or therapeutic use. Handle with standard laboratory precautions. Dispose per local regulations.
TargetCD40
HostRabbit
ClonalityPolyclonal
IsotypeIgG
ConjugateUnconjugated
ReactivityHuman, Mouse
PurificationAffinity Purification
Antigen/ImmunogenSynthesized peptide derived from Human CD40 around the phosphorylation site of T254.
ProductionThis product is a polyclonal antibody purified from rabbit antiserum.
FormLiquid
Storage BufferPBS, 50% glycerol, 0.5% BSA, 0.02% sodium azide, pH 7.4.
StorageUpon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Dilutions are starting recommendations. Titration is advised for each system.

ApplicationRecommended Dilution
Western Blot (WB)WB:1:500-1:2000, ELISA:1:5000
ELISAWB:1:500-1:2000, ELISA:1:5000

Immunogen: Synthesized peptide derived from Human CD40 around the phosphorylation site of T254.

Antigen Species: Human

Alternative Names

AI326936; B cell associated molecule CD40; B cell surface antigen CD40; B cell-associated molecule; B-cell surface antigen CD40; Bp50; CD 40; CD40; CD40 antigen (TNF receptor superfamily member 5); CD40 antigen; CD40 molecule; CD40 molecule, TNF receptor superfamily member 5; CD40 protein; CD40 type II isoform; CD40L receptor; CDw40; GP39; HIGM1; IGM; IMD3; MGC9013; Nerve growth factor receptor related B lymphocyte activation molecule; OTTHUMP00000031699; OTTHUMP00000031700; p50; T-BAM; TBAM; TNF receptor superfamily member 5; TNFRSF5; TNR5_HUMAN; TRAP; Tumor necrosis factor receptor superfamily , member 5; Tumor necrosis factor receptor superfamily member 5; Tumor necrosis factor receptor superfamily member 5 precursor; Tumor necrosis factor receptor superfamily, member 5, isoform CRA_a

What species does this CD40 antibody react with?
This antibody has been validated for reactivity against Human, Mouse. Cross-reactivity is based on the immunogen sequence and may vary by sample type.
What applications has this antibody been validated for?
Validated applications include: Western Blot (WB), ELISA. Each application was confirmed with appropriate controls. Contact technical support for applications not listed.
What is the recommended dilution?
WB:1:500-1:2000, ELISA:1:5000. Optimal concentrations depend on sample type, detection system, and antigen abundance. Titration with positive and negative controls is recommended.
How was this antibody produced?
This polyclonal IgG antibody was raised in Rabbit and purified by affinity chromatography using Protein G. It provides high specificity and minimal cross-reactivity in standard immunoassay applications.
What are the storage requirements?
Store at −20°C for long-term. Avoid repeated freeze-thaw cycles. Short-term working aliquots may be stored at 4°C. This product is supplied in a stabilized buffer containing BSA and sodium azide.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.

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