| Field | Specification |
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| Immunogen | A synthesized peptide derived from human Phospho-ErbB2(Y1221 + Y1222) was used as the immunogen for the Phospho-ErbB2 (Tyr1221/Tyr1222) antibody. |
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| Target | |
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Overview
Phospho-ErbB2 (Tyr1221/Tyr1222) Antibody / HER2 / NEU is a anti-ERBB2 (phospho-Tyr1221, Tyr1222) Rabbit antibody Recombinant Rabbit Monoclonal clone 31E43 supplied in Liquid format. Recommended for workflows such as Western blot (WB) with listed reactivity in Human.
Key elements and design rationale
- Target: ERBB2 (phospho site: Tyr1221, Tyr1222)
- Antibody details: Rabbit, Recombinant Rabbit Monoclonal, clone 31E43, isotype Rabbit IgG
- Format: Liquid
- Applications (as listed): WB
Biological background
Phospho-ErbB2 (Tyr1221/Tyr1222) antibody is widely applied in cancer biology, molecular signaling, and translational research. HER2 amplification and overexpression are defining features of aggressive breast cancer subtypes, as well as subsets of gastric, ovarian, and lung cancers. Detecting phosphorylation at Tyr1221 and Tyr1222 provides direct evidence of receptor activation, going beyond total expression levels. This allows researchers to distinguish tumors with active HER2 signaling from those with mere overexpression.
Validated applications for Phospho-ErbB2 (Tyr1221/Tyr1222) antibody include western blotting, immunohistochemistry, and immunofluorescence. In western blot assays, it identifies activated HER2 distinct from total receptor. Immunohistochemistry highlights receptor activation in tumor tissue sections, while immunofluorescence visualizes membrane localized receptor complexes in cultured cells. Together, these methods provide comprehensive tools for studying receptor activity at multiple biological scales.
Phosphorylation of HER2 at Tyr1221 and Tyr1222 is central to downstream signaling. These residues recruit adaptors that activate the Ras MAPK cascade, promoting proliferation, and the PI3K Akt pathway, enhancing survival and metabolism. Persistent phosphorylation drives oncogenesis by conferring growth advantage, resistance to apoptosis, and metastatic potential. Phospho-ErbB2 (Tyr1221/Tyr1222) antibody enables researchers to quantify this activation and study how it is regulated by dimerization partners, extracellular ligands, and therapeutic inhibitors.
HER2 targeted therapies, including trastuzumab, pertuzumab, and tyrosine kinase inhibitors such as lapatinib, directly modulate phosphorylation at these sites. Monitoring Tyr1221 and Tyr1222 phosphorylation provides a pharmacodynamic biomarker of drug response. This application is critical in translational medicine, where phospho specific antibodies can guide therapeutic development and precision oncology strategies.
HER2 signaling extends beyond cancer. It plays roles in cardiac physiology, where HER2 signaling supports cardiomyocyte survival, and in development, where it regulates tissue morphogenesis. Abnormal phosphorylation patterns may therefore impact not only tumors but also cardiac and developmental biology. Phospho-ErbB2 (Tyr1221/Tyr1222) antibody supports research across these diverse biological areas.
Phospho-ErbB2 (Tyr1221/Tyr1222) antibody from
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Quantifying post-translational regulation (including phosphorylation) alongside total protein levels.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Phospho-site readouts are condition-dependent and are often compared to total target levels when available.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
