Phospho-Histone H2A.X (Thr120) Antibody

SKU:BHA10536199
Overview
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H2AFX-specific polyclonal antibody raised in Rabbit. Validated for Western Blot in Human, Mouse, Rat samples. Affinity-purified.
Format Unconjugated
Immunogen Native Protein
Applications 1 validated
Dilution WB:1:1000-2000
Target Aliases 9 aliases
Options selector
Catalog no. Size
CSB-PA000450-50UG 50 ug
CSB-PA000450-100UG 100 ug
Available Options
Size
Field Specification
Target H2AFX
Antigen
  • Synthetic Peptide
Alternative names H2A histone family member X; H2A histone family member X; H2A.FX; H2A.X; H2a/x; H2AFX; H2AX; H2AX_HUMAN; Histone H2A.X
UniProt # P16104
Host Rabbit
Clonality
  • Polyclonal
Isotype
  • IgG
Reactivity
  • Human
  • Mouse
  • Rat
Applications
  • Western Blot
Immunogen Synthetic Peptide
Conjugate
  • Non-conjugated
Source This product is a polyclonal antibody purified from rabbit antiserum.
Purification Affinity chromatography
Form Liquid
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Storage buffer PBS, 0.02% sodium azide, 50% glycerol, pH 7.4.
Catalog no. (Mfr.) CSB-PA000450
Main SKU BHA10536199

Product Overview

Primary Antibody

This antibody targets H2AFX and was raised against a recombinant protein immunogen.

Validated Applications

Application Recommended Dilution
Western Blot (WB) WB:1:1000-2000
✔ Validated by CUSABIO Technology

Antibody Specifications

Target H2AFX
Host Rabbit
Clonality Polyclonal
Isotype IgG
Conjugate Unconjugated
Reactivity Human, Mouse, Rat
Purification Affinity Purification
Antigen / Immunogen Synthetic Peptide
Production This product is a polyclonal antibody purified from rabbit antiserum.
Form Liquid
Storage Buffer PBS, 0.02% sodium azide, 50% glycerol, pH 7.4.
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Alternative Names

H2A histone family member X; H2A histone family member X; H2A.FX; H2A.X; H2a/x; H2AFX; H2AX; H2AX_HUMAN; Histone H2A.X

Safety & Handling

⚠ Research Use Only (RUO). Not for diagnostic or therapeutic use. Handle with standard laboratory precautions. Dispose per local regulations.
TargetH2AFX
HostRabbit
ClonalityPolyclonal
IsotypeIgG
ConjugateUnconjugated
ReactivityHuman, Mouse, Rat
PurificationAffinity Purification
Antigen/ImmunogenSynthetic Peptide
ProductionThis product is a polyclonal antibody purified from rabbit antiserum.
FormLiquid
Storage BufferPBS, 0.02% sodium azide, 50% glycerol, pH 7.4.
StorageUpon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Dilutions are starting recommendations. Titration is advised for each system.

ApplicationRecommended Dilution
Western Blot (WB)WB:1:1000-2000

Immunogen: Synthetic Peptide

Antigen Species: Human

Alternative Names

H2A histone family member X; H2A histone family member X; H2A.FX; H2A.X; H2a/x; H2AFX; H2AX; H2AX_HUMAN; Histone H2A.X

What species does this H2AFX antibody react with?
This antibody has been validated for reactivity against Human, Mouse, Rat. Cross-reactivity is based on the immunogen sequence and may vary by sample type.
What applications has this antibody been validated for?
Validated applications include: Western Blot (WB). Each application was confirmed with appropriate controls. Contact technical support for applications not listed.
What is the recommended dilution?
WB:1:1000-2000. Optimal concentrations depend on sample type, detection system, and antigen abundance. Titration with positive and negative controls is recommended.
How was this antibody produced?
This polyclonal IgG antibody was raised in Rabbit and purified by affinity chromatography using Protein G. It provides high specificity and minimal cross-reactivity in standard immunoassay applications.
What are the storage requirements?
Store at −20°C for long-term. Avoid repeated freeze-thaw cycles. Short-term working aliquots may be stored at 4°C. This product is supplied in a stabilized buffer containing BSA and sodium azide.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.

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