| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | A synthesized peptide derived from human Phospho-Histone H3 (S28) was used as the immunogen for the Phospho-Histone H3 (Ser28) antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
Phospho-Histone H3 (Ser28) Antibody / HIST1H3A is an antibody targeting Phospho-Histone. Host: Rabbit. Monoclonal. Clone: 32H09. Format: Liquid. Applications: WB, IHC, ICC, IF.
Key elements and design rationale
- Target: Phospho-Histone.
- Immunogen: A synthesized peptide derived from human Phospho-Histone H3 (S28) was used as the immunogen for the Phospho-Histone H3….
- Species reactivity: Human, Mouse, Rat.
- Conjugate: Unconjugated (no label listed).
Biological background (from provided description)
Phospho-Histone H3 (Ser28) antibody detects histone H3 phosphorylated at serine 28, a dynamic modification associated with chromosome condensation and transcriptional regulation. Histone H3, encoded in part by HIST1H3A, is a core component of the nucleosome and fundamental to chromatin organization.
Common research applications
- WB: relative abundance and band pattern assessment using appropriate controls.
- IHC: tissue distribution assessment; interpret with matched controls.
- IF/ICC: subcellular localization imaging with proper background controls.
Notes for experimental interpretation
- Account for isoforms, paralogs, and post-translational modifications when interpreting signal.
- Support specificity with biological controls (e.g., KO/KD where available) and technical controls (e.g., secondary-only/isotype).
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
