| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | MAK (phospho Tyr159); MAK (phospho Y159); dJ417M14.2; Mak; MAK_HUMAN; Male germ cell associated kinase; Male germ cell-associated kinase; OTTHUMP00000016025; Rck; Serine/threonine protein kinase MAK; Serine/threonine-protein kinase MAK. |
| Conjugate | |
| Formulation | |
| Host | |
| Immunogen | KLH conjugated synthesised phosphopeptide derived from human MAK around the phosphorylation site of Tyr159:TD(p-Y)VS |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Storage |
Product Overview
phospho-MAK (Tyr159) Polyclonal Antibody detects endogenous levels of MAK protein only when phosphorylated at Y159.
The product of this gene is a serine/threonine protein kinase related to kinases involved in cell cycle regulation. It is expressed almost exclusively in the testis, primarily in germ cells. Studies of the mouse and rat homologs have localized the kinase to the chromosomes during meiosis in spermatogenesis, specifically to the synaptonemal complex that exists while homologous chromosomes are paired. There is, however, a study of the mouse homolog that has identified high levels of expression in developing sensory epithelia so its function may be more generalized.
Validated Applications
| Application | Recommended Dilution |
|---|---|
| Western Blot (WB) | See datasheet |
Product Specifications
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Species Reactivity | Human, Mouse, Rat |
| Conjugate | Unconjugated |
| Purification | affinity purified by Protein A |
| Formulation | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| Molecular Weight | ~ 73 kDa |
| Modification | Phosphorylated |
| UniProt / Swiss-Prot | P20794 |
| Storage | Store at 4°C short term. Aliquot and store at -5°C long term. Avoid freeze-thaw cycles. |
| Signaling Pathway(s) | Regulation of Microtubule Dynamics; Signaling Pathways Activating p38 MAP Kinase |
Immunogen / Antigen
KLH conjugated synthesised phosphopeptide derived from human MAK around the phosphorylation site of Tyr159:TD(p-Y)VS
Safety & Handling
For Research Use Only (RUO). Not for diagnostic or therapeutic use. Handle as a potential biohazard in accordance with good laboratory practices.
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This antibody has been validated for WB. Always refer to the product datasheet for recommended dilution ranges and appropriate positive control samples for each application type.
Validated reactivity has been confirmed for Human, Mouse, Rat. Cross-reactivity with additional species may exist based on sequence homology but has not been confirmed by the manufacturer. For unlisted species, we recommend running a preliminary validation experiment.
This antibody was raised in Rabbit. For indirect detection (WB, IHC, IF), select an anti-Rabbit secondary antibody that matches the isotype. For direct detection, check the variant selector for conjugated formats. Confirm secondary antibody compatibility before ordering.
KLH conjugated synthesised phosphopeptide derived from human MAK around the phosphorylation site of Tyr159:TD(p-Y)VS
Please refer to the storage conditions on the product label and datasheet. As a general best practice: upon receipt, aliquot into single-use volumes to minimize freeze-thaw cycling, which can progressively reduce antibody performance and lead to aggregation.
Validated reactivity covers Human, Mouse, Rat samples. Performance can vary across different cell lines and tissue types depending on target antigen expression levels and epitope accessibility. We recommend verifying target expression in your specific model system using a known positive control before proceeding to experimental samples.
BioHippo offers customization and value-added services for qualified research programs:
- Bulk Pricing: Volume discounts available for orders of 5 or more units. Request a quote through the Contact Us page.
- Custom Conjugation: Select antibody products are available for custom conjugation to fluorophores, biotin, HRP, or other detection labels upon request. Minimum quantities apply.
- Custom Formats: Lyophilized or concentrated formats may be available for select products. Inquire before ordering.
- Technical Support: Our scientific team is available to assist with application optimization, protocol troubleshooting, and experimental design.
For customization inquiries, please use the Contact Us page or reach out to our support team directly.
