Phospho-MDM2 (Tyr394) Antibody

SKU:BHA10543344
Overview
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Anti-MDM2 antibody (Rabbit, Polyclonal, IgG, Non-conjugated). Validated for Western Blot, ELISA.
Clonality Polyclonal
Host Species Rabbit
Isotype IgG
Conjugate Non-conjugated
Validated Applications Western Blot, ELISA
Purification Method Affinity Purification
Options selector
Catalog no. Size
CSB-PA455127-50UG 50 ug
CSB-PA455127-100UG 100 ug
Available Options

Select the variant that best fits your experiment. Availability and lead time may vary by option.

  • Options: Size: 50 ug, 100 ug
  • Lead time: In Stock at Manufacturer options listed in “Availability Content”; other statuses may take longer.
  • Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • Shipping: cold-chain shipment (typically with ice packs).
  • Upon receipt: store at −80°C as soon as possible.
  • Sales terms and conditions: Please review prior to ordering.
Field Specification
Target MDM2
Antigen
  • Synthetic peptide from Human protein at AA range: 330-410
Alternative names ACTFS; Double minute 2 protein; E3 ubiquitin-protein ligase Mdm2; Hdm 2; Hdm2; HDMX; MDM 2; MDM2; MDM2 oncogene E3 ubiquitin protein ligase; Mdm2 p53 E3 ubiquitin protein ligase homolog; Mdm2 transformed 3T3 cell double minute 2 p53 binding protein (mouse) binding protein 104kDa; MDM2_HUMAN; MDM2BP; Mouse Double Minute 2; MTBP; Murine Double Minute Chromosome 2; Oncoprotein Mdm2; p53 Binding Protein Mdm2; p53-binding protein Mdm2; Ubiquitin protein ligase E3 Mdm2; Ubiquitin protein ligase E3 Mdm2
UniProt # Q00987
Host Rabbit
Clonality
  • Polyclonal
Isotype
  • IgG
Reactivity
  • Human
  • Mouse
Applications
  • Western Blot
  • ELISA
Immunogen Synthetic peptide from Human protein at AA range: 330-410
Conjugate
  • Non-conjugated
Source This product is a polyclonal antibody purified from rabbit antiserum.
Purification Antigen affinity purification
Form Liquid
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Storage buffer PBS, 0.02% sodium azide, 50% glycerol, pH 7.4.
Catalog no. (Mfr.) CSB-PA455127
Main SKU BHA10543344

Product Overview

Research Use Only

MDM2 is a research-grade antigen target for which this antibody provides selective detection in commonly studied model systems.

Validated Applications

Western Blot (WB) 1:500-2000
ELISA (ELISA) 1:10000-20000

Key Specifications

Clonality Polyclonal
Host Species Rabbit
Isotype IgG
Conjugate Non-conjugated
Purification Antigen affinity purification
Catalog No. CSB-PA455127
Alternative Names ACTFS; Double minute 2 protein; E3 ubiquitin-protein ligase Mdm2; Hdm 2; Hdm2; HDMX; MDM 2; MDM2; MDM2 oncogene E3 ubiquitin protein ligase; Mdm2 p53 E3 ubiquitin protein ligase homolog; Mdm2 transformed 3T3 cell double minute 2 p53 binding protein (mouse) binding protein 104kDa; MDM2_HUMAN; MDM2BP; Mouse Double Minute 2; MTBP; Murine Double Minute Chromosome 2; Oncoprotein Mdm2; p53 Binding Protein Mdm2; p53-binding protein Mdm2; Ubiquitin protein ligase E3 Mdm2; Ubiquitin protein ligase E3 Mdm2

Immunogen / Antigen

Synthetic peptide from Human protein at AA range: 330-410 (Antigen species: Human)

Storage & Handling

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Safety

For Research Use Only (RUO). Not intended for diagnostic, therapeutic, or veterinary use. Handle according to standard laboratory biosafety guidelines. Avoid skin and eye contact. Dispose of waste in accordance with local regulations.

What applications has the Phospho-MDM2 (Tyr394) Antibody been validated for?

This antibody has been validated for: Western Blot, ELISA. For each application, follow the recommended dilutions on the product datasheet and optimize empirically for your sample type.

What is the recommended dilution range?

WB:1:500-2000, ELISA:1:10000-20000

Which species does this antibody recognize?

This antibody has been confirmed reactive against: the species listed on the datasheet. Cross-reactivity with other species may occur due to sequence homology but is not guaranteed without independent validation.

What is the immunogen / antigen used to generate this antibody?

Synthetic peptide from Human protein at AA range: 330-410

How should this antibody be stored and handled?

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. Avoid repeated freeze-thaw cycles. Centrifuge vials briefly before opening. Dilute immediately before use.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.

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