Phospho-MUSK (Tyr755) Antibody

Overview

This is a polyclonal anti-MUSK antibody raised in Rabbit, with confirmed utility in ELISA, WB. It is designed to detect MUSK protein in Human, Mouse, Rat and supports researchers working in signal transduction contexts.

Key elements and design rationale

• Immunogen: Peptide sequence around phosphorylation site of tyrosine 755(A-D-Y(p)-Y-K) derived from Human MuSK . — determines the epitope region; confirm compatibility with sample preparation and expected post-translational modifications.
• Host species (Rabbit): Requires anti-rabbit-IgG secondary reagents for indirect detection.
• Polyclonal format: Recognizes multiple epitopes for robust signal; inherent lot-to-lot variability requires appropriate controls.
• Isotype (IgG): Matched secondary antibodies and isotype controls required. Compatible with standard Protein A/G purification workflows.
• Purification (Antigen affinity purification): Enriches for specific immunoglobulin classes; reduces non-specific background vs. crude antisera.

Biological background

MUSK (also referred to as CMS9, FADS, MDK 4, MDK4, MGC126323, MGC126324, Muscle, Muscle associated receptor tyrosine kinase, Muscle skeletal receptor tyrosine kinase, Muscle skeletal receptor tyrosine protein kinase, Muscle specific kinase receptor, Muscle specific tyrosine kinase receptor, Muscle specific tyrosine protein kinase receptor, Muscle-specific kinase receptor, Muscle-specific tyrosine-protein kinase receptor, MuSK, MUSK_HUMAN, Neural fold somite kinase 1, Neural fold somite kinase 2, Neural fold somite kinase 3, Neural fold somite kinase1, Neural fold somite kinase2, Neural fold somite kinase3, Nsk 1, Nsk 2, Nsk 3, Nsk1, Nsk2, Nsk3, Receptor tyrosine kinase MuSK, Skeletal muscle receptor tyrosine kinase, skeletal receptor tyrosine-protein kinase) is a protein target studied in Human systems. Expression, subcellular localization, and post-translational modifications vary across cell types and disease states — factors critical to antibody-based detection design. Consult UniProt, NCBI Gene, and primary literature for current annotation of MUSK biology in signal transduction.

Common research applications

• ELISA: Quantification of soluble target in biological fluids or culture supernatants. Ensure samples are within the linear detection range.
• WB: Confirms target molecular weight and distinguishes isoforms under denaturing conditions.

Notes for experimental interpretation

• Isotype controls: Use an isotype-matched (IgG from Rabbit) control at equivalent concentration to assess non-specific background.
• Cross-reactivity: Polyclonal preparations may cross-react with related proteins. Orthogonal validation (siRNA, KO lysate) is recommended.
• Matrix effects: Sample matrix can affect performance; pilot dilution linearity and spike-recovery experiments are advised for quantitative studies.
• Species reactivity: Confirmed for Human, Mouse, Rat. Extrapolation to untested species requires empirical validation.

This product is a polyclonal antibody purified from rabbit antiserum.

• Datasheet (11084658)

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.