Phospho-PIK3R1/PIK3R3 (Y467/199) Antibody

SKU:BHA10539005
Overview
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PIK3R1/PIK3R3-specific polyclonal antibody raised in Rabbit. Validated for Western Blot, Immunohistochemistry, Immunofluorescence in Human, Mouse, Rat samples. Affinity-purified.
Format Unconjugated
Immunogen Native Protein
Applications 4 validated
Dilution WB:1:500-1:2000, IHC:1:100-1:300, IF:1:2
Target Aliases 7 aliases
Options selector
Catalog no. Size
CSB-PA030058-50UG 50 ug
CSB-PA030058-100UG 100 ug
Available Options
Size
Field Specification
Target PIK3R1/PIK3R3
Antigen
  • Synthesized peptide derived from Human PI 3-kinase p85/p55 around the phosphorylation site of Y467/199.
Alternative names PIK3R1; GRB1; Phosphatidylinositol 3-kinase regulatory subunit alpha; PI3-kinase regulatory subunit alpha; PI3K regulatory subunit alpha; PtdIns-3-kinase regulatory subunit alpha; Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alph
UniProt # P27986/Q92569
Host Rabbit
Clonality
  • Polyclonal
Isotype
  • IgG
Reactivity
  • Human
  • Mouse
  • Rat
  • Monkey
Applications
  • Western Blot
  • Immunohistochemistry
  • Immunofluorescence
  • ELISA
Immunogen Synthesized peptide derived from Human PI 3-kinase p85/p55 around the phosphorylation site of Y467/199.
Conjugate
  • Non-conjugated
Source This product is a polyclonal antibody purified from rabbit antiserum.
Purification Antigen affinity purification
Form Liquid
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Storage buffer PBS, 50% glycerol, 0.5% BSA, 0.02% sodium azide, pH 7.4.
Catalog no. (Mfr.) CSB-PA030058
Main SKU BHA10539005

Product Overview

Primary Antibody

This antibody targets PIK3R1/PIK3R3 and was raised against a recombinant protein immunogen.

Validated Applications

Application Recommended Dilution
Western Blot (WB) WB:1:500-1:2000, IHC:1:100-1:300, IF:1:200-1:1000, ELISA:1:10000
Immunohistochemistry (IHC) WB:1:500-1:2000, IHC:1:100-1:300, IF:1:200-1:1000, ELISA:1:10000
Immunofluorescence (IF) WB:1:500-1:2000, IHC:1:100-1:300, IF:1:200-1:1000, ELISA:1:10000
ELISA WB:1:500-1:2000, IHC:1:100-1:300, IF:1:200-1:1000, ELISA:1:10000
✔ Validated by CUSABIO Technology

Antibody Specifications

Target PIK3R1/PIK3R3
Host Rabbit
Clonality Polyclonal
Isotype IgG
Conjugate Unconjugated
Reactivity Human, Mouse, Rat, Monkey
Purification Affinity Purification
Antigen / Immunogen Synthesized peptide derived from Human PI 3-kinase p85/p55 around the phosphorylation site of Y467/199.
Production This product is a polyclonal antibody purified from rabbit antiserum.
Form Liquid
Storage Buffer PBS, 50% glycerol, 0.5% BSA, 0.02% sodium azide, pH 7.4.
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Alternative Names

PIK3R1; GRB1; Phosphatidylinositol 3-kinase regulatory subunit alpha; PI3-kinase regulatory subunit alpha; PI3K regulatory subunit alpha; PtdIns-3-kinase regulatory subunit alpha; Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alph

Safety & Handling

⚠ Research Use Only (RUO). Not for diagnostic or therapeutic use. Handle with standard laboratory precautions. Dispose per local regulations.
TargetPIK3R1/PIK3R3
HostRabbit
ClonalityPolyclonal
IsotypeIgG
ConjugateUnconjugated
ReactivityHuman, Mouse, Rat, Monkey
PurificationAffinity Purification
Antigen/ImmunogenSynthesized peptide derived from Human PI 3-kinase p85/p55 around the phosphorylation site of Y467/199.
ProductionThis product is a polyclonal antibody purified from rabbit antiserum.
FormLiquid
Storage BufferPBS, 50% glycerol, 0.5% BSA, 0.02% sodium azide, pH 7.4.
StorageUpon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Dilutions are starting recommendations. Titration is advised for each system.

ApplicationRecommended Dilution
Western Blot (WB)WB:1:500-1:2000, IHC:1:100-1:300, IF:1:200-1:1000, ELISA:1:10000
Immunohistochemistry (IHC)WB:1:500-1:2000, IHC:1:100-1:300, IF:1:200-1:1000, ELISA:1:10000
Immunofluorescence (IF)WB:1:500-1:2000, IHC:1:100-1:300, IF:1:200-1:1000, ELISA:1:10000
ELISAWB:1:500-1:2000, IHC:1:100-1:300, IF:1:200-1:1000, ELISA:1:10000

Immunogen: Synthesized peptide derived from Human PI 3-kinase p85/p55 around the phosphorylation site of Y467/199.

Antigen Species: Human

Alternative Names

PIK3R1; GRB1; Phosphatidylinositol 3-kinase regulatory subunit alpha; PI3-kinase regulatory subunit alpha; PI3K regulatory subunit alpha; PtdIns-3-kinase regulatory subunit alpha; Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alph

What species does this PIK3R1/PIK3R3 antibody react with?
This antibody has been validated for reactivity against Human, Mouse, Rat, Monkey. Cross-reactivity is based on the immunogen sequence and may vary by sample type.
What applications has this antibody been validated for?
Validated applications include: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA. Each application was confirmed with appropriate controls. Contact technical support for applications not listed.
What is the recommended dilution?
WB:1:500-1:2000, IHC:1:100-1:300, IF:1:200-1:1000, ELISA:1:10000. Optimal concentrations depend on sample type, detection system, and antigen abundance. Titration with positive and negative controls is recommended.
How was this antibody produced?
This polyclonal IgG antibody was raised in Rabbit and purified by affinity chromatography using Protein G. It provides high specificity and minimal cross-reactivity in standard immunoassay applications.
What are the storage requirements?
Store at −20°C for long-term. Avoid repeated freeze-thaw cycles. Short-term working aliquots may be stored at 4°C. This product is supplied in a stabilized buffer containing BSA and sodium azide.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.

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