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| Immunogen | A synthesized peptide derived from human Phospho-PKC (S729) was used as the immunogen for the Phospho-PKC (Ser729) antibody. |
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Overview
Phospho-PKC (Ser729) Antibody / PRKCA/PRKCB/PRKCE is a anti-PKC (phospho-Ser729) Rabbit antibody Recombinant Rabbit Monoclonal clone 32P75 supplied in Liquid format. Recommended for workflows such as Western blot (WB) with listed reactivity in Human, Rat.
Key elements and design rationale
- Target: PKC (phospho site: Ser729)
- Antibody details: Rabbit, Recombinant Rabbit Monoclonal, clone 32P75, isotype Rabbit IgG
- Format: Liquid
- Applications (as listed): WB
Biological background
Phospho-PKC (Ser729) antibody is widely applied in cancer biology, signal transduction, and neuroscience. PKC enzymes regulate cell proliferation, differentiation, apoptosis, and cytoskeletal remodeling. Phosphorylation at Ser729 serves as a marker for mature active PKC isoforms and is required for proper subcellular localization and substrate phosphorylation. By detecting phospho Ser729, researchers can measure activation status of PKC pathways and how they change in disease states.
Western blot assays reveal distinct phospho PKC bands corresponding to activated isoforms. Immunohistochemistry maps Ser729 phosphorylation in tissues such as brain, heart, and tumors. Immunofluorescence highlights dynamic translocation of PKC from cytosol to plasma membrane or other compartments upon stimulation. These methods allow precise characterization of PKC activation at the molecular and cellular level.
PKC activity is critical for oncogenic signaling. Dysregulation of phosphorylation at Ser729 is associated with altered PKC stability and oncogenic transformation. Certain cancers show reduced PKC activity due to mutations preventing proper phosphorylation, while others display hyperactivation that promotes tumor growth. By applying Phospho-PKC (Ser729) antibody, scientists can study how phosphorylation events regulate tumor biology and therapeutic response.
PKC enzymes are also central to neuronal signaling and plasticity. Phosphorylation at Ser729 supports synaptic transmission, long term potentiation, and memory formation. Altered PKC activity has been implicated in neurodegenerative diseases such as Alzheimer disease and Parkinson disease. With phospho specific antibody tools, researchers can dissect how PKC phosphorylation influences brain function and pathology.
In cardiology, PKC phosphorylation regulates cardiac hypertrophy, contractility, and response to ischemia. The Ser729 site provides a biomarker for assessing PKC function in cardiac stress and adaptation. This makes Phospho-PKC (Ser729) antibody relevant not only for molecular signaling studies but also for translational research into heart disease.
Phospho-PKC (Ser729) antibody from
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Quantifying post-translational regulation (including phosphorylation) alongside total protein levels.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Phospho-site readouts are condition-dependent and are often compared to total target levels when available.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
