Phospho-PRAS40 (Thr246) Antibody / AKT1S1

Phospho-PRAS40 (Thr246) antibody recognizes a phosphorylated form of the proline-rich AKT substrate of 40 kDa, encoded by the AKT1S1 gene. PRAS40 is a critical signaling adaptor that links the AKT pathway with the mechanistic target of rapamycin complex 1 (mTORC1). Under normal conditions, PRAS40 binds to mTORC1 and functions as an inhibitor, but phosphorylation of PRAS40 at threonine 246 by AKT reduces this inhibitory effect. This phosphorylation event facilitates activation of mTORC1, which drives protein synthesis, cell growth, and metabolic reprogramming. Because of this central role, detection with Phospho-PRAS40 (Thr246) antibody is important for studies exploring insulin signaling, cancer progression, and metabolic regulation.PRAS40 phosphorylation is tightly regulated by upstream PI3K-AKT activation. Following growth factor stimulation, AKT phosphorylates PRAS40 at Thr246, creating a binding site for 14-3-3 proteins. The resulting complex alters PRAS40 localization and diminishes its inhibitory function on mTORC1. Dysregulated phosphorylation at this site has been observed in multiple cancers, including breast and prostate carcinoma, where increased AKT signaling is a hallmark. Phospho-PRAS40 (Thr246) antibody therefore provides a valuable tool for researchers assessing therapeutic inhibitors of the PI3K-AKT-mTOR pathway.Beyond oncology, Thr246 phosphorylation has been linked to metabolic diseases. In insulin-resistant states, abnormal AKT-PRAS40 signaling alters glucose uptake and lipid metabolism. Detecting this modification with Phospho-PRAS40 (Thr246) antibody can clarify molecular mechanisms underlying type 2 diabetes and obesity. It has also been used to monitor experimental therapies designed to restore insulin sensitivity.Phospho-PRAS40 (Thr246) antibody is widely applied in western blotting, immunohistochemistry, and ELISA-based studies. In western blotting, phosphorylation-specific detection distinguishes the activated protein from the unmodified form, providing precise pathway analysis. Immunohistochemistry enables visualization of phosphorylated PRAS40 within tissue architecture, revealing spatial relationships with other signaling components. ELISA or bead-based assays further allow quantitative comparisons of phosphorylation across experimental conditions.PRAS40 phosphorylation often occurs alongside other AKT targets, such as GSK3, FOXO, and TSC2. Using Phospho-PRAS40 (Thr246) antibody in parallel with other phospho-specific antibodies allows comprehensive mapping of AKT activity in cellular models.