Phospho-RPS6KB1 (T421+S424) Antibody

SKU:BHA10564577
Overview
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RPS6KB1-specific monoclonal antibody raised in Rabbit. Validated for ELISA, Western Blot, Immunofluorescence in Human samples. Affinity-purified.
Format Unconjugated
Immunogen Native Protein
Applications 4 validated
Dilution WB:1:500-1:5000, IF:1:20-1:200, IP:1:200
Target Aliases 1 aliases
Options selector
Catalog no. Size
CSB-RA020470A421phHU-50UL 50 uL
CSB-RA020470A421phHU-100UL 100 uL
Available Options
Size
Field Specification
Target RPS6KB1
Antigen
  • A synthesized peptide derived from human Phospho-RPS6KB1 (T421+S424)
Alternative names Ribosomal protein S6 kinase beta-1, 70 kDa ribosomal protein S6 kinase 1, P70S6K1, p70-S6K 1, Ribosomal protein S6 kinase I, Serine/threonine-protein kinase 14A, p70 ribosomal S6 kinase alpha, p70 S6 kinase alpha, p70 S6K-alpha, p70 S6KA, RPS6KB1, STK14A
UniProt # P23443
Host Rabbit
Clone 3B6
Clonality
  • Monoclonal
Isotype
  • IgG
Reactivity
  • Human
Applications
  • ELISA
  • Western Blot
  • Immunofluorescence
  • Immunoprecipitation
Immunogen A synthesized peptide derived from human Phospho-RPS6KB1 (T421+S424)
Conjugate
  • Non-conjugated
Source This product is a recombinant monoclonal antibody expressed in HEK293F cells.
Purification Affinity chromatography
Form Liquid
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Storage buffer PBS, 150mM NaCl, 50% Glycerol, 0.05% BSA, 0.02% Sodium Azide, pH 7.4.
Catalog no. (Mfr.) CSB-RA020470A421phHU
Main SKU BHA10564577

Product Overview

Primary Antibody

This antibody targets RPS6KB1 and was raised against a recombinant protein immunogen.

Validated Applications

Application Recommended Dilution
ELISA WB:1:500-1:5000, IF:1:20-1:200, IP:1:200-1:1000
Western Blot (WB) WB:1:500-1:5000, IF:1:20-1:200, IP:1:200-1:1000
Immunofluorescence (IF) WB:1:500-1:5000, IF:1:20-1:200, IP:1:200-1:1000
Immunoprecipitation (IP) WB:1:500-1:5000, IF:1:20-1:200, IP:1:200-1:1000
✔ Validated by CUSABIO Technology

Antibody Specifications

Target RPS6KB1
Host Rabbit
Clonality Monoclonal
Isotype IgG
Conjugate Unconjugated
Reactivity Human
Purification Affinity Purification
Antigen / Immunogen A synthesized peptide derived from human Phospho-RPS6KB1 (T421+S424)
Clone Number 3B6
Production This product is a recombinant monoclonal antibody expressed in HEK293F cells.
Form Liquid
Storage Buffer PBS, 150mM NaCl, 50% Glycerol, 0.05% BSA, 0.02% Sodium Azide, pH 7.4.
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Alternative Names

Ribosomal protein S6 kinase beta-1, 70 kDa ribosomal protein S6 kinase 1, P70S6K1, p70-S6K 1, Ribosomal protein S6 kinase I, Serine/threonine-protein kinase 14A, p70 ribosomal S6 kinase alpha, p70 S6 kinase alpha, p70 S6K-alpha, p70 S6KA, RPS6KB1, STK14A

Safety & Handling

⚠ Research Use Only (RUO). Not for diagnostic or therapeutic use. Handle with standard laboratory precautions. Dispose per local regulations.
TargetRPS6KB1
HostRabbit
ClonalityMonoclonal
IsotypeIgG
ConjugateUnconjugated
ReactivityHuman
PurificationAffinity Purification
Antigen/ImmunogenA synthesized peptide derived from human Phospho-RPS6KB1 (T421+S424)
Clone Number3B6
ProductionThis product is a recombinant monoclonal antibody expressed in HEK293F cells.
FormLiquid
Storage BufferPBS, 150mM NaCl, 50% Glycerol, 0.05% BSA, 0.02% Sodium Azide, pH 7.4.
StorageUpon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Dilutions are starting recommendations. Titration is advised for each system.

ApplicationRecommended Dilution
ELISAWB:1:500-1:5000, IF:1:20-1:200, IP:1:200-1:1000
Western Blot (WB)WB:1:500-1:5000, IF:1:20-1:200, IP:1:200-1:1000
Immunofluorescence (IF)WB:1:500-1:5000, IF:1:20-1:200, IP:1:200-1:1000
Immunoprecipitation (IP)WB:1:500-1:5000, IF:1:20-1:200, IP:1:200-1:1000

Immunogen: A synthesized peptide derived from human Phospho-RPS6KB1 (T421+S424)

Antigen Species: Human

Alternative Names

Ribosomal protein S6 kinase beta-1, 70 kDa ribosomal protein S6 kinase 1, P70S6K1, p70-S6K 1, Ribosomal protein S6 kinase I, Serine/threonine-protein kinase 14A, p70 ribosomal S6 kinase alpha, p70 S6 kinase alpha, p70 S6K-alpha, p70 S6KA, RPS6KB1, STK14A

What species does this RPS6KB1 antibody react with?
This antibody has been validated for reactivity against Human. Cross-reactivity is based on the immunogen sequence and may vary by sample type.
What applications has this antibody been validated for?
Validated applications include: ELISA, Western Blot (WB), Immunofluorescence (IF), Immunoprecipitation (IP). Each application was confirmed with appropriate controls. Contact technical support for applications not listed.
What is the recommended dilution?
WB:1:500-1:5000, IF:1:20-1:200, IP:1:200-1:1000. Optimal concentrations depend on sample type, detection system, and antigen abundance. Titration with positive and negative controls is recommended.
How was this antibody produced?
This monoclonal IgG antibody was raised in Rabbit and purified by affinity chromatography using Protein G. It provides high specificity and minimal cross-reactivity in standard immunoassay applications.
What are the storage requirements?
Store at −20°C for long-term. Avoid repeated freeze-thaw cycles. Short-term working aliquots may be stored at 4°C. This product is supplied in a stabilized buffer containing BSA and sodium azide.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.

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