| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | A synthesized peptide derived from human Phospho-Src (Y529) was used as the immunogen for the Phospho-Src (Tyr529) antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
Phospho-Src (Tyr529) Antibody is an antibody targeting Phospho-Src. Host: Rabbit. Monoclonal. Clone: 32S01. Format: Liquid. Applications: WB, ICC, IF.
Key elements and design rationale
- Target: Phospho-Src.
- Immunogen: A synthesized peptide derived from human Phospho-Src (Y529) was used as the immunogen for the Phospho-Src (Tyr529) anti….
- Species reactivity: Human, Mouse, Rat.
- Conjugate: Unconjugated (no label listed).
Biological background (from provided description)
Phospho-Src (Tyr529) antibody detects Src kinase phosphorylated at tyrosine 529, a regulatory modification that maintains Src in an inactive conformation. Src is a non-receptor tyrosine kinase involved in diverse cellular pathways, including proliferation, adhesion, and cytoskeletal dynamics.
Common research applications
- WB: relative abundance and band pattern assessment using appropriate controls.
- IF/ICC: subcellular localization imaging with proper background controls.
Notes for experimental interpretation
- Account for isoforms, paralogs, and post-translational modifications when interpreting signal.
- Support specificity with biological controls (e.g., KO/KD where available) and technical controls (e.g., secondary-only/isotype).
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
