| Field | Specification |
|---|---|
| Target | |
| Alternative names | DHS; Fam38a; Family with sequence similarity 38 member A; KIAA0233; Membrane protein induced by beta-amyloid treatment; Mib; PIEZ1_HUMAN; Piezo-type mechanosensitive ion channel component 1; PIEZO1; Protein FAM38A; Protein FAM38B; Protein PIEZO1 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | Recombinant Human Piezo-type mechanosensitive ion channel component 1 protein (2230-2420AA) |
| Conjugate | |
| Source | This product is a polyclonal antibody purified from rabbit antiserum. |
| Purification | |
| Form | Liquid |
| Storage | |
| Storage buffer | |
| Catalog no. (Mfr.) | |
| Main SKU |
Overview
This is a polyclonal anti-PIEZO1 antibody raised in Rabbit, with confirmed utility in ELISA, IHC. It is designed to detect PIEZO1 protein in Human and supports researchers working in tags & cell markers contexts where consistent antibody performance is required.
Key elements and design rationale
- Immunogen: Recombinant Human Piezo-type mechanosensitive ion channel component 1 protein (2230-2420AA) — the immunizing antigen determines the epitope region; confirm epitope compatibility with sample preparation and expected post-translational modifications.
- Host species (Rabbit): Requires anti-rabbit-IgG secondary reagents. Use matched secondaries to avoid no-signal or cross-reactivity issues.
- Polyclonal format: Recognizes multiple epitopes, providing robust signal across varied preparations and species variants. Inherent lot-to-lot variability requires appropriate controls.
- Isotype (IgG): Compatible with standard Protein A/G purification and widely supported by secondary reagents. Include an isotype-matched control at equivalent concentration.
- Purification (Antigen affinity purification): Enriches for specific immunoglobulin classes, reducing non-specific populations and improving signal-to-noise.
Biological background
PIEZO1 (also referred to as DHS, Fam38a, Family with sequence similarity 38 member A, KIAA0233, Membrane protein induced by beta-amyloid treatment, Mib, PIEZ1_HUMAN, Piezo-type mechanosensitive ion channel component 1, PIEZO1, Protein FAM38A, Protein FAM38B, Protein PIEZO1) is a protein target studied in Human systems. Expression levels, subcellular localization, and post-translational modifications vary across cell types, tissues, and disease states — factors that influence antibody-based detection and experimental design. Consult UniProt, NCBI Gene, and primary literature for current annotation of PIEZO1 biology, including known isoforms, interactors, and disease-relevant expression patterns in tags & cell markers.
Common research applications
- ELISA: Sandwich or indirect ELISA can quantify soluble target in biological fluids or culture supernatants. Ensure samples fall within the linear detection range.
- IHC: IHC visualizes target in FFPE or frozen tissue sections. Optimize antigen retrieval method and secondary detection for each tissue type.
Notes for experimental interpretation
- Isotype controls: Use an isotype-matched (IgG from Rabbit) control at equivalent concentration to assess non-specific background.
- Cross-reactivity: Polyclonal preparations may cross-react with related proteins or isoforms. Orthogonal validation (siRNA, KO lysate, recombinant protein) is recommended to confirm signal specificity.
- Matrix effects: Sample matrix (serum, plasma, lysate, homogenate) can affect performance. Pilot dilution linearity and spike-recovery experiments are recommended for quantitative studies.
- Species reactivity: Confirmed for Human. Extrapolation to untested species requires empirical validation given potential epitope sequence divergence.
This product is a polyclonal antibody purified from rabbit antiserum.
PIEZO1 is a mechanically activated (mechanosensitive) cation channel that converts mechanical force into ion flux.
It is a Rabbit polyclonal antibody.
This antibody is validated for ELISA and immunohistochemistry (IHC). Optimal dilutions should be determined by the end user for each application.
Predicted/tested reactivity: human. Confirm reactivity for your species and sample type with appropriate controls.
No. This antibody is for in vitro research use only (RUO); it is not intended for diagnostic or therapeutic use in humans or animals.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
