| Field | Specification |
|---|---|
| Target | |
| Alternative names | tcag7.927; BG; FLJ39445; MPS7; beta-D-glucuronidase |
| UniProt # | |
| Species | |
| Applications | |
| Assay type | |
| Sensitivity | |
| Detection range | |
| Catalog no. (Mfr.) | |
| Main SKU |
Scientific Background
Beta-glucuronidase is an enzyme that in humans is encoded by the GUSB gene.Human beta-glucuronidase is synthesized as an 80 kDa monomer (653 amino acids) before proteolysis removes 18 amino acids from the C-terminal end to form a 78 kDa monomer. Biologically, beta-glucuronidase exists as a 332 kDa homotetramer. Beta-glucuronidase contains several notable structural formations, including a type of beta barrel known as a jelly roll barrel and a TIM barrel. Human beta-glucuronidase is homologous to the Escherichia coli enzyme beta-galactosidase. This homologous relationship, along with the knowledge that glycosidases often perform hydrolysis catalyzed by two acidic residues, enabled the development of a mechanistic hypothesis.
Assay Principle
This assay employs a two-site sandwich ELISA to quantitate GUSB in samples. An antibody specific for GUSB has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyGUSB present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GUSB is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GUSB bound in the initial step. The color development is stopped and the intensity of the color is measured.
Performance Data
| Assay Type | Sandwich ELISA |
|---|---|
| Detection Range | 62.5-4000 pg/mL |
| Sensitivity | 27.4 pg/mL |
| Species Reactivity | Pig (Sus scrofa; Porcine) |
| Stability | The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C). |
| Manufacturer No. | AE60903PI |
| UniProt # | Q4FAT7 |
| Alternative Names | tcag7.927; BG; FLJ39445; MPS7; beta-D-glucuronidase |
Assay Workflow
The kit includes the components required for standard-curve quantification (see the kit manual for the component table and validated sample types). Absorbance is read on a standard microplate reader and concentrations are interpolated from the standard curve.
Safety & Handling
For research use only (RUO). Not for use in diagnostic or therapeutic procedures. Handle all samples and reagents using standard laboratory safety practices.
Related Products
Explore additional Pig ELISA kits from Wuhan Abebio Science Co Ltd, or contact us for related assays for GUSB research.
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