| Field | Specification |
|---|---|
| Target | |
| Alternative names | FLJ10761; HNFJ2; angiotensin-forming enzyme|angiotensinogenase|renin precursor; renal |
| UniProt # | |
| Species | |
| Applications | |
| Assay type | |
| Sensitivity | |
| Detection range | |
| Catalog no. (Mfr.) | |
| Main SKU |
Scientific Background
Renin is a member of the aspartyl proteinase family produced largely in part by the juxtaglomerular cells in the kidney.Renin differs from the other members of this class by having a pH optimum near neutral pH region with native substrates instead of 2.0 to 3.4 This more neutral pH optimum allows it to be functional in the plasma. Renin also has very high selectivity for substrate due to quite long peptide recognition on either side of the peptide bond undergoing cleavage. An octapeptide substrate was the minimum length to be cleaved by renin. Renin plays a crucial role in the regulation of blood pressure and salt balance through the cleavage of angiotensinogen, which is the only known physiological substrate of renin.
Assay Principle
This assay employs a two-site sandwich ELISA to quantitate REN in samples. An antibody specific for REN has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyREN present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for REN is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of REN bound in the initial step. The color development is stopped and the intensity of the color is measured.
Performance Data
| Assay Type | Sandwich ELISA |
|---|---|
| Detection Range | 62.5-4000 pg/mL |
| Sensitivity | 1.56 pg/mL |
| Species Reactivity | Pig (Sus scrofa; Porcine) |
| Stability | The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C). |
| Manufacturer No. | AE22488PI |
| UniProt # | Q2KIS1 |
| Alternative Names | FLJ10761; HNFJ2; angiotensin-forming enzyme|angiotensinogenase|renin precursor; renal |
Assay Workflow
The kit includes the components required for standard-curve quantification (see the kit manual for the component table and validated sample types). Absorbance is read on a standard microplate reader and concentrations are interpolated from the standard curve.
Safety & Handling
For research use only (RUO). Not for use in diagnostic or therapeutic procedures. Handle all samples and reagents using standard laboratory safety practices.
Related Products
Explore additional Pig ELISA kits from Wuhan Abebio Science Co Ltd, or contact us for related assays for REN research.
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