| Field | Specification |
|---|---|
| Mfr No | |
| Product Type | |
| Reporter | |
| Selection Marker | Blasticidin, Hygromycin, Puromycin, Zeocin |
| Shipping | |
| Species |
Background
Pit-1 (pituitary-specific positive transcription factor 1, encoded by POU1F1) is a POU-domain transcription factor essential for development and function of the anterior pituitary. Expressed predominantly in somatotrope and lactotrope cells, Pit-1 activates the genes encoding growth hormone and prolactin and is required for the differentiation of these hormone-producing lineages. Loss-of-function mutations in POU1F1 cause combined pituitary hormone deficiency. Pit-1 is also expressed in the mammary gland, where its overexpression has been associated with breast cancer progression, extending its biological significance beyond the pituitary and making it a relevant target in endocrinology research.
Product Description & Applications
The PitRE Reporter Lentivirus is a transcription-factor reporter system for detecting Pit-1 (POU1F1) transcriptional activity. It contains tandem repeats of Pit-1 response elements (PitRE) derived from the prolactin promoter, coupled to a minimal TATA-box promoter and an upstream enhancer that maximizes signal-to-noise to drive a fluorescent or luminescent reporter (including BFP2, d2GFP, EGFP, GFP, mCherry, RFP, firefly, Gaussia, or Renilla luciferase). A constitutively expressed selection marker (blasticidin, hygromycin, puromycin, or zeocin) supports stable polyclonal cell line generation. Stable lentiviral integration provides consistent reporter expression in dividing and post-mitotic cells, including primary and cryopreserved material, with readout by microscopy, flow cytometry, or luminometry. It is used to study Pit-1 activity in endocrinology and pituitary biology research. Supplied as purified lentiviral particles.
About This Product
This reporter lentivirus places a BFP2, d2GFP, EGFP, Firefly Luc, Gaussia Luc, GFP, GFP + Firefly Luc, mCherry, Renilla Luc, RFP, RFP + Firefly Luc reporter gene under the control of tandem consensus response elements specific for the Pit-1/PouF1 transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Blasticidin, Hygromycin, Puromycin, Zeocin) and/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.
Stable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.
Can't find the lentiviral construct you need, or want to adjust key design elements? Contact us to discuss custom LV design and optional add-ons.
Common customization requests
- Insert / payload: replace the gene/sequence, swap to a different isoform, add mutations, or optimize cloning features.
- Expression design: change promoter (e.g., CMV/EF1α/PGK), add enhancers, or adjust regulatory elements.
- Reporters: add/swap GFP/RFP/mCherry/luciferase (single or dual reporters where applicable).
- Selection markers: add/swap puromycin/blasticidin/neomycin or fluorescent selection options.
- Vector format: switch between OE, shRNA, CRISPR (sgRNA/Cas systems), or control vectors (where supported).
Add-ons you can request
- Control viruses: empty vector, non-targeting shRNA, reporter-only controls, or matched backbone controls.
- Packaging / format: concentration options, aliquoting, or custom fill volume for screening workflows.
- Documentation: construct map/sequence confirmation package (as available) and batch documentation.
What to include in your request
- Target cell type/model (cell line or primary cells) and intended readout (reporter, knockdown, OE, etc.)
- Insert sequence (FASTA) or reference ID, plus any required tags/mutations
- Promoter, reporter, and selection marker preferences
- Desired scale and preferred format (aliquots / concentration requests)
Email us at support@biohippo.com or use the Talk to a Scientist request form.
