| Field | Specification |
|---|---|
| Applications | |
| Storage | |
| Shelf life | |
| Catalog no. (Mfr.) | |
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Product Overview
The Plant/Fungal DNA Extraction Kit isolates and purifies total DNA from 100–500 mg of fresh plant tissue, 20–50 mg of dried plant tissue or 100–300 mg of fungi. The DNA is eluted in 100–200 µL of preheated Buffer TE. The expected purity is about OD260/OD280 1.8–2.0.
Samples are lysed at 65°C for 30 min, extracted with chloroform at 12,000 rpm for 5 min and precipitated with isopropanol. The mixture is bound to adsorption column P at 12,000 rpm for 30 s, washed once with Buffer RPB and twice with Wash Buffer WB, dried at 14,000 rpm for 1 min and eluted with preheated Buffer TE after standing 2 min at room temperature.
Specifications
| Format | Spin column (adsorption column P) |
|---|---|
| Sample types | Fresh plant tissue, dried plant tissue and fungi |
| Sample input | 100–500 mg fresh plant tissue, 20–50 mg dried plant tissue, or 100–300 mg fungi |
| Purity (OD260/OD280) | About 1.8–2.0 |
| Elution volume | 100–200 µL preheated Buffer TE |
| Special treatment | RNase A supplied |
Kit Components
| Component | 50 T | 200 T |
|---|---|---|
| RNase A | 275 µL | 1.1 mL |
| Buffer PGE | 60 mL | 240 mL |
| Buffer RPB | 30 mL | 120 mL |
| Wash Buffer WB | 60 mL | 240 mL |
| Buffer TE | 10 mL | 40 mL |
| Adsorption column P | 50 set | 200 set |
Applications
- Total DNA extraction from fresh plant tissue
- Total DNA extraction from dried plant tissue
- Genomic DNA extraction from fungi
Storage & Handling
Buffers PGE, RPB, WB and TE and the adsorption columns are stored at room temperature for 1 year. RNase A is stored at -20°C for 1 year.
Usage Notes
- Avoid repeated freeze-thaw cycles of the sample.
- Lyse the sample fully to prevent the column from blocking.
- For low yield, increase the sample amount proportionally within the stated range.
- If precipitation occurs in a buffer, pre-incubate it at 37°C before use.
- Confirm that ethanol has been added to the wash buffers before use.
- Allow 2–5 minutes for the DNA to dissolve during elution for best results.
- Chloroform and isopropanol are required but are not supplied with the kit.
Safety & Regulatory
For research use only. Not intended for clinical, diagnostic or therapeutic use. Follow the manufacturer's manual and standard laboratory safety practice.
Customization & Add-ons: Need this molecular biology reagent tailored to your workflow? We can help with bulk or custom pack sizes, alternative formats (spin column, magnetic bead or reagent-only kits; premixed or stand-alone reference dye), matched extraction, cDNA synthesis and qPCR workflows, and sourcing of related enzymes, ladders and consumables not in our catalog. Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services — our team will follow up with feasibility details and next steps.
