| Field | Specification |
|---|---|
| Applications | |
| Storage | |
| Shelf life | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This kit uses a unique buffer system and is especially suitable for extracting genomic DNA from fresh plant materials. It can remove impurities, proteins and other organic compounds in cells to the greatest extent. The kit yields high-molecular-weight genomic DNA of high purity. The DNA recovered by this kit can be used in various routine operations, including enzyme digestion, PCR, library construction, Southern hybridization and so on.
Plant tissue of 100 mg is ground under liquid nitrogen and lysed in 600 µL of Buffer PGE for 20 min at 60°C. The lysate is extracted with 700 µL of chloroform and centrifuged at 12,000 rpm for 10 min; DNA in the aqueous phase is precipitated with isopropanol, washed twice with 75% ethanol, air-dried and dissolved in 50–200 µL of Elution Buffer. Purified DNA has an OD260/OD280 of 1.7–1.9.
Specifications
| Format | Solution-based extraction with chloroform and isopropanol precipitation |
|---|---|
| Sample type | Fresh plant material, a variety of plant tissues |
| Sample input | 100 mg fresh tissue or 20 mg dry weight |
| Purity | OD260/OD280 should be 1.7–1.9 |
| Processing time | Within 1 hour |
| Elution volume | 50–200 µL Elution Buffer |
Kit Components
| Component | 50 T | 200 T |
|---|---|---|
| Buffer PGE | 30 mL | 120 mL |
| Elution Buffer | 10 mL | 40 mL |
Applications
- Genomic DNA extraction from fresh plant tissue
- Enzyme digestion and PCR
- Library construction
- Southern hybridization
Key Features
- Simple and fast: High-quality genomic DNA can be obtained within 1 hour.
- Wide application: It can be applied to a variety of tissues of a variety of plants.
Storage & Handling
All components are stored at room temperature for 1 year.
Usage Notes
- Avoid repeated freezing and thawing to prevent DNA fragmentation.
- Buffer PGE may turn yellow, which does not affect the extraction result.
- Chloroform, isopropanol and 75% ethanol are used in the protocol but are not supplied in the kit; handle them in a fume hood.
- Air-dry the residual ethanol before dissolving the DNA.
- A larger dissolution volume increases yield but lowers the DNA concentration.
Safety & Regulatory
For research use only. Not intended for clinical, diagnostic or therapeutic use. Follow the manufacturer's manual and standard laboratory safety practice.
Customization & Add-ons: Need this molecular biology reagent tailored to your workflow? We can help with bulk or custom pack sizes, alternative formats (spin column, magnetic bead or reagent-only kits; premixed or stand-alone reference dye), matched extraction, cDNA synthesis and qPCR workflows, and sourcing of related enzymes, ladders and consumables not in our catalog. Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services — our team will follow up with feasibility details and next steps.
