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Product Overview
The ground plant tissue is dissolved by the strong lysis solution and filtered to remove protein impurities and cell debris. Column purification technology efficiently removes residual protein and PCR inhibitors. After RNA is washed with solution RRPB and Solution RWB, it is eluted with RNase-Free ddH₂O, which can be used in various molecular biology experiments.
A 100 mg sample is ground and lysed in 600 µL of Lysis buffer C, clarified at 12,000 rpm for 5 min, mixed with 0.3 volumes of isopropanol and loaded onto the R adsorption column at 10,000 rpm for 1 min. The column is washed with 500 µL of Solution RRPB, treated on-column with 80 µL of DNase I working solution for 10 min at room temperature, washed again with RRPB and twice with 500 µL of Wash Buffer RWB, dried at 12,000 rpm for 2 min and eluted with 30–100 µL of RNase-Free ddH₂O. The expected purity is OD260/OD280 1.8–2.1, with 2.0 indicating high-quality RNA.
Specifications
| Format | Spin column (adsorption column R) |
|---|---|
| Sample type | Plant tissue |
| Sample input | 100 mg standard; 150 mg for fruits, tubers and petals; 60–100 mg for dried seeds |
| Chemistry | No phenol/chloroform extraction, cesium chloride gradient or lithium chloride/ethanol precipitation |
| Special treatment | On-column DNase I; DNase I stock solution and DNase I Buffer RDB supplied |
| Purity (OD260/OD280) | 1.8–2.1; 2.0 indicates high-quality RNA |
| Elution volume | 30–100 µL RNase-Free ddH₂O |
Kit Components
| Component | 50 T | 200 T |
|---|---|---|
| Lysis buffer C | 50 mL | 200 mL |
| RNA deproteinizing Solution RRPB | 60 mL | 240 mL |
| DNase I stock solution | 525 µL | 2.1 mL |
| DNase I Buffer RDB | 4 mL | 20 mL |
| Wash Buffer RWB | 60 mL | 240 mL |
| Adsorption Column R | 50 sets | 200 sets |
| RNase-Free ddH₂O | 40 mL | 160 mL |
Applications
- Total RNA extraction from plant tissue
- RNA extraction from fruits, tubers, petals and dried seeds
- Removal of DNA contamination with on-column DNase I
- Downstream molecular biology experiments requiring high-purity RNA
Key Features
- Formulated specifically for plant material, so the workflow is simpler than a general-purpose RNA protocol.
- Equipped with DNase I to remove DNA contamination.
- RNA has higher purity and no impurity residues, which is especially suitable for downstream experiments that require high purity.
- The operation is safe and reliable, no phenol/chloroform extraction, no cesium chloride gradient centrifugation, no lithium chloride or ethanol precipitation.
Storage & Handling
Most components are stored at room temperature for 1 year; the DNase I stock solution is stored at -20°C for 1 year.
Usage Notes
- Add absolute ethanol to Wash Buffer RWB before use.
- If the lysis buffer precipitates, dissolve it at 60–65°C before use.
- Do not shake vigorously when mixing with isopropanol.
- Dry the column thoroughly; residual rinse solution interferes with downstream reactions.
- Store the RNA solution at -70°C.
Safety & Regulatory
For research use only. Not intended for clinical, diagnostic or therapeutic use. Follow the manufacturer's manual and standard laboratory safety practice.
Customization & Add-ons: Need this molecular biology reagent tailored to your workflow? We can help with bulk or custom pack sizes, alternative formats (spin column, magnetic bead or reagent-only kits; premixed or stand-alone reference dye), matched extraction, cDNA synthesis and qPCR workflows, and sourcing of related enzymes, ladders and consumables not in our catalog. Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services — our team will follow up with feasibility details and next steps.
