| Field | Specification |
|---|---|
| Applications | |
| Storage | |
| Shelf life | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
In this kit, plasmid DNA is purified rapidly by modified SDS alkaline lysis combined with selective adsorption of DNA on a DNA preparation membrane. It applies to extract up to 20 µg of high purity plasmid DNA from 1–5 mL bacterial culture. It has been used in molecular biology experiments such as sequencing, in vitro transcription and translation, restriction endonuclease digestion and bacterial transformation.
Bacteria are cultured for 12–16 h at 37°C and lysed with Solutions A, B and C, and the cleared lysate is loaded onto Adsorption column P. The column is washed twice with 600 µL of Wash Buffer and the plasmid DNA is eluted in 30–100 µL of Elution Buffer, with 50 µL recommended. Purified plasmid DNA has an OD260/OD280 of about 1.8–2.0.
Specifications
| Format | Spin column (Adsorption column P) |
|---|---|
| Lysis method | Modified SDS alkaline lysis |
| Sample type | Bacterial culture |
| Sample input | 1–5 mL bacterial culture |
| Yield | Up to 20 µg plasmid DNA |
| Recovery | More than 85% of plasmid DNA from bacteria |
| Purity | OD260/OD280 is about 1.8–2.0 |
| Elution volume | 30–100 µL, 50 µL recommended |
Kit Components
| Component | 50 T | 200 T |
|---|---|---|
| RNase A | 75 µL | 300 µL |
| Solution A | 15 mL | 60 mL |
| Solution B | 15 mL | 60 mL |
| Solution C | 15 mL | 60 mL |
| Wash Buffer | 60 mL | 240 mL |
| Elution Buffer | 5 mL | 20 mL |
| Adsorption column P | 50 set | 200 set |
Applications
- Plasmid preparation for sequencing
- In vitro transcription and translation
- Restriction endonuclease digestion
- Bacterial transformation
Key Features
- Fast: Few steps, simple operation, save time.
- High efficiency: More than 85% of plasmid DNA can be extracted from bacteria.
Storage & Handling
All components are stored at room temperature for 1 year, except RNase A, which is stored at -20°C for 1 year. Solution A is stored at 4°C once RNase A has been added to it.
Usage Notes
- Add RNase A to Solution A before first use and store Solution A at 4°C thereafter.
- Culture bacteria for 12–16 h at 37°C before extraction.
- Stand the column for 2 min before elution.
- Apply Elution Buffer at 56°C and stand 5 min before the final 2 min centrifugation.
Safety & Regulatory
For research use only. Not intended for clinical, diagnostic or therapeutic use. Follow the manufacturer's manual and standard laboratory safety practice.
Customization & Add-ons: Need this molecular biology reagent tailored to your workflow? We can help with bulk or custom pack sizes, alternative formats (spin column, magnetic bead or reagent-only kits; premixed or stand-alone reference dye), matched extraction, cDNA synthesis and qPCR workflows, and sourcing of related enzymes, ladders and consumables not in our catalog. Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services — our team will follow up with feasibility details and next steps.
Itaconate inhibits ferroptosis of macrophage via Nrf2 pathways against sepsis-induced acute lung injury. Cell Death Discovery, 2022. 10.1038/s41420-021-00807-3
