| Field | Specification |
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| Host | |
| Immunogen | A synthesized peptide derived from human Pleiotrophin was used as the immunogen for the Pleiotrophin antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
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Overview
Pleiotrophin Antibody / PTN is a anti-PTN Rabbit antibody Recombinant Rabbit Monoclonal clone 32P54 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC) with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasm, cell membrane.
Key elements and design rationale
- Target: PTN
- Antibody details: Rabbit, Recombinant Rabbit Monoclonal, clone 32P54, isotype Rabbit IgG
- Format: Liquid
- Applications (as listed): WB, IHC
Biological background
Pleiotrophin antibody is applied in developmental biology, oncology, and regenerative medicine research. The protein interacts with receptors such as receptor protein tyrosine phosphatase beta zeta, syndecans, and anaplastic lymphoma kinase, activating signaling cascades including PI3K AKT and MAPK. These pathways promote proliferation and survival, making pleiotrophin an important regulator of both physiological and pathological growth. Detecting pleiotrophin provides insight into how extracellular cues influence signaling and cell fate decisions.
The antibody is validated for western blotting, immunohistochemistry, immunofluorescence, and ELISA. In western blot assays, pleiotrophin antibody identifies bands corresponding to the growth factor across tissue extracts. Immunohistochemistry reveals expression in vasculature and developing tissues, while immunofluorescence highlights extracellular distribution and receptor colocalization. ELISA applications allow quantification of secreted pleiotrophin in culture media or patient samples.
Overexpression of pleiotrophin has been reported in cancers including glioblastoma, breast carcinoma, and prostate cancer. In these contexts, pleiotrophin enhances angiogenesis, invasion, and tumor progression. By applying pleiotrophin antibody, scientists can evaluate pleiotrophin levels as a biomarker and investigate its potential as a therapeutic target.
In neuroscience, pleiotrophin plays roles in neuronal survival, synaptic plasticity, and axonal regeneration after injury. It promotes neurite outgrowth and supports recovery in models of neural damage. In skeletal biology, pleiotrophin regulates osteoblast differentiation and bone growth, linking it to both normal development and fracture healing. These diverse functions demonstrate the broad research value of pleiotrophin antibody.
Pleiotrophin antibody provided by
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
