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(±)12(13)-DiHOME is the vicinal diol metabolite of (±)12(13)-EpOME (i.e., isoleukotoxin), an epoxide derived from linoleic acid via cytochrome P450 epoxygenase activity. It has been shown to suppress neutrophil respiratory burst activity and exhibit selective cytotoxicity in certain cell lines expressing sEH. Thus, (±)12(13)-DiHOME bridges lipid metabolism and immune modulation, acting both as a downstream stress-responsive signal and as a paracrine regulator in inflammation and energy homeostasis.
Assay Principle
Intended Use The Tribioscience’s (±)12(13)-DiHOME ELISA Kit utilizes competitive ELISA technologies for the quantitative analysis of (±)12(13)-DiHOME in biological samples. It provides a vital tool to accurately measure (±)12(13)-DiHOME with applications in inflammation, metabolism, and respiratory research. The assay has a range of 0.14-100 ng/ml.
Typical Data This standard curve is provided for demonstration only. A standard curve should be generated for each set of samples assayed. Fig. 3 is an example of typical Data.
Simple Procedures (Fig. 1) Simple Standard Preparation
(±)12(13)-DiHOME ELISA Kit is a Competitive ELISA for the quantitative determination of (±)12(13)-DiHOME. In this competitive ELISA, sample analyte competes with a labeled competitor for a limited number of antibody binding sites, so signal is inversely proportional to analyte concentration.
Kit Components
The kit provides a pre-coated microplate, target standard for standard-curve generation, detection reagents (detection antibody and enzyme conjugate), wash buffer, substrate, and stop solution. Refer to the product datasheet for the complete component list and quantities.
Performance Data
| Target | (±)12(13)-DiHOME |
|---|---|
| Assay Type | Competitive |
| Test Principle | Competitive ELISA |
| Detection Range | 0.14–100 ng/mL |
| Storage | Store the unopened kit at 2-8°C. |
✓ Quality tested for reproducibility and lot-to-lot consistency
Safety & Handling
This assay has a detection range of 0.14–100 ng/mL. Samples reading outside the range should be diluted or concentrated.
The kit provides a pre-coated microplate, target standard for standard-curve generation, detection reagents (detection antibody and enzyme conjugate), wash buffer, substrate, and stop solution. See the datasheet for the full component list and quantities.
A standard microplate reader capable of measuring absorbance at 450 nm is required for this competitive ELISA.
Customization & Add-ons: Need this ELISA kit tailored to your workflow? We can help with bulk or custom pack sizes, species or analyte variants, matched antibody pairs and standards, alternate plate formats (48- or 96-well, strip-well), and assay development or custom ELISA solutions for targets not in our catalog. Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services — our team will follow up with feasibility details and next steps.
