| Field | Specification |
|---|---|
| Mfr No | |
| Activity | |
| Cas No. | |
| Concentration | |
| Form | Lyophilized powder. |
| Product Type | |
| Purity | |
| Reconstitution | |
| Shipping | |
| Solubility | DMSO or methanol. Centrifuge all product preparations before use (10000 x g 5 min). |
| Source | Semi-synthetic |
| Storage | |
| Target |
Product details
- Chemical name: (1aR,1bS,4aR,7aS,7bS,8R,9R,9aS)-9a-(Acetyloxy)- 1a,1b,4, 4a,5,7a,7b,8,9,9a-decahydro-4a,7b-dihydroxy-3-(hydroxymethyl)-1,1,6,8-tetramethyl-5-oxo-1H-cyclopropa[3,4]benz [1,2-e]azulen-9-yl tetradecanoate.
- Also known as: Phorbol 12-myristate 13-acetate, TPA, 12-O-tetradecanoylphorbol-13-acetate
- CAS number: 16561-29-8
- Molecular formula: C36H56O8.
- Purity: >99.5%
- Concentration: 200 nM.
- Form: Lyophilized powder.
- Solubility: DMSO or methanol. Centrifuge all product preparations before use (10000 x g 5 min).
- Reconstitution: Centrifuge the vial before adding solvent (10,000 x g for 5 minutes) to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity
- Shipping: Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
- Target: PKC
- Source: Semi-synthetic
- Activity: PMA is a commonly used phorbol ester and activates PKC in nM concentrations1. It has also been shown to be an extremely potent tumor promoter2-4.
- Alternative names: Phorbol 12-myristate 13-acetate, TPA, 12-O-tetradecanoylphorbol-13-acetate
Scientific background
Phorbol 12-myristate 13-acetate (PMA) is the most commonly-used phorbol ester, primary found as extremely potent tumor promoter.1-3 PMA is a reversible activator of Protein Kinase C (PKC)4 in vivo and in vitro, even at nM concentrations (cells are usually treated with PMA at 200 nM to induce signaling). PMA promotes the expression of inducible NOS in cultured hepatocytes, activates Ca2+-ATPase pump and potentates forskolin-induced cAMP formation. In some cases PMA inhibits apoptosis induced by the Fas antigen, but induces apoptosis in HL-60 promyelocytic leukemia cells.5-8
Lead time: 1-2 Business Days
Country of origin: Israel/IL
Applications key
Application key: FC- Flow cytometry, IFC- Indirect flow cytometry, IHC- Immunohistochemistry,LCI- Live cell imaging, Calcium imaging assay,Cell survival assay, Electrophysiology, Neurite outgrowth assay.
Bioassay tested: Yes
Need this compound in a format that drops straight into your assay? We can tailor formulation, chemistry, and documentation so your results stay consistent across runs and re-orders.
- Format options: solid or pre-dissolved solution (choose solvent), target concentration, aliquots, light/moisture-protected packaging
- Chemistry options: free base/acid vs salt forms, hydrate/solvate preference, stereoisomer control (single enantiomer or racemate), close analogs
- Add-on labels & handles: D/¹³C/¹⁵N isotopes (LC-MS/internal standards), azide/alkyne or other functional handles for conjugation
- QC & documentation: standard COA or enhanced analytical pack (HPLC/LC-MS/NMR), chiral purity, residual solvents, water content (KF), method-specific specs
- Scale & continuity: mg to gram scale, bulk pricing, lot reservation, repeat-order continuity
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