| Field | Specification |
|---|---|
| Applications | |
| Sterility | Negative for bacteria, yeast, fungi, and mycoplasma. |
| Cell type | |
| Form | Liquid |
| Storage | |
| Shipping | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Podocytes are terminally differentiated highly specialized epithelial cells that lining the outer surface of the glomerular capillaries in kidney [1]. Podocytes together with the opposing monolayers of fenestrated endothelial cells in the vascular space and glomerular basement membrane (GBM) in between form the three-layer glomerular filtration barrier which functions as size-selective and charge-dependent molecular sieve for the main function of nephron. Podocytes injury, which is usually termed “foot process effacement” is a pathological process found in many kidney diseases [2]. In vitro cultured podocytes are useful model to study podocytes biology. These cells can also be used with glomerular endothelial cells for coculture studies in an attempt to better study barrier permeability or be cultured in Matrigel for three-dimensional or organoid studies[3].
Podocytes Culture Medium is a sterile-filtered, ready-to-use liquid medium from iXCells Biotechnologies for the routine culture of Podocytes in vitro. Each lot is screened for bacteria, yeast, fungi and mycoplasma before release.
Scientific Background
iXCells® Podocytes Culture Medium is a complete medium uniquely designed to maintain primary podocyte growth in vitro. The cells maintain their cell specific markers as well as function for as long as 2-3 passages which makes it suitable for the following cell characterization and functional assays. The medium contains all the necessary nutrients that podocyte needs to grow and perform their function. The medium is provided as the antibiotics free complete culture medium supplemented with the working concentration of fetal bovine serum (FBS). No additional FBS is needed for cell culture.
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Specifications
| Catalog No. | MD-0120 |
|---|---|
| Package Size | 500 mL |
| Form | Liquid |
| Sterility | Negative for bacteria, yeast, fungi, and mycoplasma. |
| Storage | Store at -20°C. Protect from light. |
| Shipping | Dry ice |
| Country of Manufacture | United States of America (USA) |
Note: Please store the medium immediately at -20°C upon arrival.
Product Details
- Mammalian cells
- Primary cells
- With serum (Fetal bovine serum)
- With phenol red
- Antibiotics free
QC & Validation
Lot-release testing
Tested in the iXCells Human Podocytes (Cat. 10HU-250) following the recommended protocol in a 37°C, 5% CO2 incubator.
Usage Notes
Do not use components that are beyond the expiration date indicated on the label. Tested in the iXCells Human Podocytes (Cat: 10HU-250) following the recommended protocol in a 37 °C, 5% CO2 incubator. Not for use in Diagnostic procedures. Results may vary due to variations among species, organ or tissue origin, and donors. Results may vary due to different culture conditions including temperature, CO2 concentration, culture vessels, coating conditions, medium adding amount, and medium change frequence. For certain culture or test conditions, please follow the culture protocol for each specific cell type. iXCells in house cell culture test was performed under antibiotics free condition for ≥ 3 days by following the recommended protocol, and no bacterial or fungi contamination were observed during the culture period. Optional: Antibiotics-Antimycotic (Fisher, Cat# 15240062) or Penicillin Streptomycin (Thermo Fisher, Cat# 15140122) at working solution could be added if necessary, which have been tested in house without visible effect on cell morphology or function.
Safety & Handling: When handling biohazardous materials such as cryopreserved cells, safe laboratory procedures should be followed, and personal protective equipment should be worn including but not limited to face masks, gloves, and lab coat.
Storage & Handling
For laboratory research use only. It is not approved for human or animal use.
Customization & Add-ons: Need this medium in a format that fits your protocol—or a formulation we don’t list? We can help you source the best match or support custom culture media for diverse research needs, including basal medium and complete medium formats, serum options (defined FBS lot, low-serum, serum-free or xeno-free), antibiotic preferences (antibiotic-free, Penicillin-Streptomycin or Antibiotic-Antimycotic), phenol red–free formulations, supplement and growth-factor adjustments (concentration changes, separate supplement packs, custom component kits), fill volume and packaging (small aliquots through bulk volumes), and lot reservation and repeat-order continuity for longitudinal studies (project dependent). We can also help you pair a medium with matched primary cells, coating reagents and dissociation solutions. Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
