| Field | Specification |
|---|---|
| Mfr No | |
| Enzyme Type | |
| Expression System | |
| Form | Liquid |
| Formulation | |
| Product Type | |
| Purity | |
| Source | E.coli. |
| Species | |
| Storage | |
| Target |
Product Description
Polymerase II Polypeptide F Human Recombinant
Overview
Polymerase II Polypeptide F (POLR2F) is the 6th largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes, which is also shared by the other two DNA-directed RNA polymerases. In yeast, POLR2F, in combination with at least 2 other subunits, forms a structure which stabilizes the transcribing polymerase on the DNA template. DNA-dependent RNA polymerases catalyze the transcription of DNA into RNA via the 4 ribonucleoside triphosphates as substrates. Pol II is the principal element of the basal RNA polymerase II transcription apparatus. Pols are comprised of mobile elements which move close to each other. In Pol II, POLR2F/RPB6 is part of the clamp element and along with parts of RPB1 and RPB2 constructs a compartment to which the RPB4-RPB7 subcomplex attaches.
Specifications
| Purity | Greater than 95% as determined by SDS-PAGE. |
|---|---|
| Formulation | The POLR2F solution (1mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl, 20% glycerol and 1mM DTT. |
| Storage | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Expression System | E.coli. |
