| Field | Specification |
|---|---|
| Target | |
| Alternative names | DNA-directed RNA polymerase III subunit RPC4 (RNA polymerase III subunit C4)|DNA-directed RNA polymerase III subunit D|Protein BN51|RNA polymerase III 47 kDa subunit|RPC53 homolog|POLR3D|BN51|BN51T |
| Gene ID | |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | polymerase(RNA) III(DNA directed) polypeptide D, 44kDa |
| Conjugate | |
| Molecular weight | |
| Purity | |
| Purification | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Scientific Background
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific peripheric component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs(EBERs) induce type I interferon and NF-Kappa-B through the RIG-I pathway(By similarity).
Product Overview
This polyclonal antibody, raised in Rabbit, recognizes POLR3D. It is validated for ELISA, Western Blot.
Validated Applications
| Applications | ELISA, Western Blot |
|---|---|
| WB | 1:500-1:2000 |
Optimal dilutions should be determined by the end user for each application and sample type.
Immunogen
polymerase(RNA) III(DNA directed) polypeptide D, 44kDa
Specifications
| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse, Rat |
| Immunogen | polymerase(RNA) III(DNA directed) polypeptide D, 44kDa |
| Purification | Immunogen Affinity Purified |
| Purity | ≥95% as determined by SDS-PAGE |
| Form | Liquid |
| Target MW | 44 kDa |
| UniProt # | P05423 |
| Gene ID | 661 |
| Storage | PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.) |
| Alternative Names | DNA-directed RNA polymerase III subunit RPC4 (RNA polymerase III subunit C4)|DNA-directed RNA polymerase III subunit D|Protein BN51|RNA polymerase III 47 kDa subunit|RPC53 homolog|POLR3D|BN51|BN51T |
Reactivity Notes
Validated reactivity: Human, Mouse, Rat. For other species, epitope conservation may allow cross-reactivity; confirm with appropriate controls.
Safety & Handling
This product is for research use only (RUO) and is not intended for diagnostic or therapeutic use. The formulation contains sodium azide; handle with standard laboratory precautions.
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Explore related antibodies and reagents for POLR3D research from Fine Test, or contact us for alternative formats.
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